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Product Info Summary
| SKU: | A05299-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-FATP4/SLC27A4 Antibody Picoband®
View all FATP4/SLC27A4 Antibodies
SKU/Catalog Number
A05299-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FATP4/SLC27A4 Antibody Picoband® catalog # A05299-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-FATP4/SLC27A4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05299-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FATP4/SLC27A4 recombinant protein (Position: K15-L643).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05299-2 is reactive to SLC27A4 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
39411 MW
Background of FATP4/SLC27A4
Long-chain fatty acid transport protein 4 is a protein that in humans is encoded by the SLC27A4 gene. This gene encodes a member of a family of fatty acid transport proteins, which are involved in translocation of long-chain fatty acids cross the plasma membrane. This protein is expressed at high levels on the apical side of mature enterocytes in the small intestine, and appears to be the principal fatty acid transporter in enterocytes. Clinical studies suggest this gene as a candidate gene for the insulin resistance syndrome. Mutations in this gene have been associated with ichthyosis prematurity syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05299-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human 293T whole cell, rat brain tissue, rat testis tissue, rat kidney tissue, mouse testis tissue
IHC: human colorectal adenocarcinoma tissue, human prostatic carcinoma tissue, human rectum adenocarcinoma tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FATP4/SLC27A4 using anti-FATP4/SLC27A4 antibody (A05299-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat testis tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FATP4/SLC27A4 antigen affinity purified polyclonal antibody (Catalog # A05299-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FATP4/SLC27A4 at approximately 70 kDa. The expected band size for FATP4/SLC27A4 is at 72 kDa.
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Figure 2. IHC analysis of FATP4/SLC27A4 using anti-FATP4/SLC27A4 antibody (A05299-2).
FATP4/SLC27A4 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FATP4/SLC27A4 Antibody (A05299-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of FATP4/SLC27A4 using anti-FATP4/SLC27A4 antibody (A05299-2).
FATP4/SLC27A4 was detected in a paraffin-embedded section of human prostatic carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FATP4/SLC27A4 Antibody (A05299-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 4. IHC analysis of FATP4/SLC27A4 using anti-FATP4/SLC27A4 antibody (A05299-2).
FATP4/SLC27A4 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FATP4/SLC27A4 Antibody (A05299-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SLC27A4 (Source: Uniprot.org, NCBI)
Gene Name
SLC27A4
Full Name
Long-chain fatty acid transport protein 4
Weight
39411 MW
Superfamily
ATP-dependent AMP-binding enzyme family
Alternative Names
Loricrin; LOR; LRN SLC27A4 ACSVL4, FATP4, IPS solute carrier family 27 member 4 long-chain fatty acid transport protein 4|arachidonate--CoA ligase|long-chain-fatty-acid--CoA ligase|solute carrier family 27 (fatty acid transporter), member 4|very long-chain acyl-CoA synthetase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC27A4, check out the SLC27A4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC27A4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FATP4/SLC27A4 Antibody Picoband® (A05299-2)
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