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Product Info Summary
| SKU: | PB9252 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Fas Antibody Picoband®
SKU/Catalog Number
PB9252
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fas Antibody Picoband® catalog # PB9252. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fas Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9252)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Fas recombinant protein (Position: Q26-N173). Human Fas shares 55% and 59% amino acid (aa) sequences identity with mouse and rat Fas, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9252 is reactive to FAS in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
37732 MW
Background of FAS
FAS (also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. FAS antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor (TNF) receptors and the low-affinity nerve growth factor receptor (NGFR) and it is mapped to 10q24.1. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9252 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, JURKAT Whole Cell, A549 Whole Cell, SMMC Whole Cell, K562 Whole Cell
IHC: Human Lung Cancer Tissue
Validation Images & Assay Conditions
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Anti-FAS Picoband antibody, PB9252, Western blotting
All lanes: Anti FAS (PB9252) at 0.5ug/ml
WB: Recombinant Human FAS Protein 0.5ng
Predicted bind size: 35KD
Observed bind size: 35KD
Click image to see more details
Anti-FAS Picoband antibody, PB9252, Western blotting
All lanes: Anti FAS (PB9252) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: K562 Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
Click image to see more details
Anti-FAS Picoband antibody, PB9252, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For FAS (Source: Uniprot.org, NCBI)
Gene Name
FAS
Full Name
Tumor necrosis factor receptor superfamily member 6
Weight
37732 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 6;Apo-1 antigen;Apoptosis-mediating surface antigen FAS;FASLG receptor;CD95;FAS;APT1, FAS1, TNFRSF6; FAS ALPS1A, APO-1, APT1, CD951, FASTM, TNFRSF6, FAS Fas cell surface death receptor tumor necrosis factor receptor superfamily member 6|APO-1 cell surface |CD95 |FASLG receptor|Fas (TNF receptor superfamily, member 6)|Fas AMA|TNF receptor superfamily member 6|apoptosis 1|apoptosis signaling receptor FAS|apoptosis-mediating surface FAS|mutant tumor necrosis receptor superfamily member 6|tumor necrosis factor receptor superfamily, member 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FAS, check out the FAS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FAS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fas Antibody Picoband® (PB9252)
Hello CJ!
PB9252 has been cited in 24 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Significance of Fas and FasL protein expression in cardiac carcinoma and local lymph node tissues
Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues
The effect of geniste on Aβ25–35-induced PC12 cell apoptosis through the JNK-dependent Fas pathway
Role of Cyt-C/caspases-9,3, Bax/Bcl-2 and the FAS death receptor pathway in apoptosis induced by zinc oxide nanoparticles in human aortic endothelial cells and the protective effect by alpha-lipoic acid
Ferguson Bennit HR,Gonda A,Kabagwira J,Oppegard L,Chi D,Licero Campbell J,De Leon M,Wall NR.Natural Killer Cell Phenotype and Functionality Affected by Exposure to Extracellular Survivin and Lymphoma-Derived Exosomes.Int J Mol Sci.2021 Jan 27;22(3):1255.d
Species: Human
PB9252 usage in article: APP:FLOW CYTOMETRY, SAMPLE:EXOSOMES, DILUTION:NA
Osteoprotegerin Induces Apoptosis of Osteoclasts and Osteoclast Precursor Cells via the Fas/Fas Ligand Pathway
HSP70 inhibits stress-induced cardiomyocyte apoptosis by competitively binding to FAF1
Construction and identification of Fas-targeting siRNA-expressing plasmid
Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells
Significance of Fas and FasL protein expression in cardiac carcinoma and local lymph node tissues
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Customer Q&As
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16 Customer Q&As for Anti-Fas Antibody Picoband®
Question
I was wanting to use to test anti-Fas antibody PB9252 on human right ovary for research purposes, then I may be interested in using anti-Fas antibody PB9252 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
The products we sell, including anti-Fas antibody PB9252, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-28
Question
Would PB9252 anti-Fas antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
You can see on the product datasheet, PB9252 anti-Fas antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-24
Question
Is this PB9252 anti-Fas antibody reactive to the isotypes of FAS?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The immunogen of PB9252 anti-Fas antibody is E.coli-derived human Fas recombinant protein (Position: Q26-N173). Human Fas shares 55% and 59% amino acid (aa) sequences identity with mouse and rat Fas, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-28
Question
We bought anti-Fas antibody for WB on cervix carcinoma last year. I am using human, and We intend to use the antibody for IHC next. you antibody examining cervix carcinoma as well as urinary bladder in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
I looked at the website and datasheets of our anti-Fas antibody and I see that PB9252 has been validated on human in both WB and IHC. Thus PB9252 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-22
Question
I was wanting to use your anti-Fas antibody for WB for human right ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human right ovary identification?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
It shows on the product datasheet, PB9252 anti-Fas antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-23
Question
Please see the WB image, lot number and protocol we used for right ovary using anti-Fas antibody PB9252. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-13
Question
Do you have a BSA free version of anti-Fas antibody PB9252 available?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Fas antibody PB9252 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-21
Question
Will anti-Fas antibody PB9252 work for WB with right ovary?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
According to the expression profile of right ovary, FAS is highly expressed in right ovary. So, it is likely that anti-Fas antibody PB9252 will work for WB with right ovary.
Boster Scientific Support
Answered: 2019-09-26
Question
I am looking for using your anti-Fas antibody for fasl/ cd95l signaling studies. Has this antibody been tested with western blotting on a549 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
I appreciate your inquiry. This PB9252 anti-Fas antibody is tested on hela whole cell lysate, jurkat whole cell lysate, a549 whole cell lysate, smmc whole cell lysate, k562 whole cell lysate, lung cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-28
Question
My question regarding product PB9252, anti-Fas antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9252 anti-Fas antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-Fas antibody PB9252. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-30
Question
We are currently using anti-Fas antibody PB9252 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
The anti-Fas antibody (PB9252) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-05
Question
Is a blocking peptide available for product anti-Fas antibody (PB9252)?
T. Yang
Verified customer
Asked: 2017-06-07
Answer
We do provide the blocking peptide for product anti-Fas antibody (PB9252). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-07
Question
My team were satisfied with the WB result of your anti-Fas antibody. However we have seen positive staining in peripheral blood lymphocyte isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is FAS supposed to be expressed?
K. Jackson
Verified customer
Asked: 2016-02-03
Answer
Based on literature, peripheral blood lymphocyte does express FAS. Generally FAS expresses in isoform 1: cell membrane, isoform 2: secreted., isoform 3: secreted., isoform 4: secreted., isoform 5: secreted., isoform 6: secreted. Regarding which tissues have FAS expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Liver, Pubmed ID: 19159218, 24275569
Peripheral blood lymphocyte, Pubmed ID: 14702039
Urinary bladder, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-02-03
Question
I see that the anti-Fas antibody PB9252 works with WB, what is the protocol used to produce the result images on the product page?
V. Krishna
Verified customer
Asked: 2014-07-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-07-01
Question
We have observed staining in human cervix carcinoma. Do you have any suggestions? Is anti-Fas antibody supposed to stain cervix carcinoma positively?
J. Brown
Verified customer
Asked: 2014-06-19
Answer
According to literature cervix carcinoma does express FAS. According to Uniprot.org, FAS is expressed in right ovary, peripheral blood lymphocyte, urinary bladder, cervix carcinoma, liver, among other tissues. Regarding which tissues have FAS expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Liver, Pubmed ID: 19159218, 24275569
Peripheral blood lymphocyte, Pubmed ID: 14702039
Urinary bladder, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-06-19
