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Product Info Summary
| SKU: | PA1119 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Fas Antibody Picoband®
SKU/Catalog Number
PA1119
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fas Antibody catalog # PA1119. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fas Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1119)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of rat Fas.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1119 is reactive to Fas in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
12 kDa
Calculated molecular weight
36835 MW
Background of FAS
FAS (also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors, can induce apoptosis or, conversely, can deliver growth stimulatory signals. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor (TNF) receptors and the low-affinity nerve growth factor receptor (NGFR) and is mapped to 10q24.1. And the FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1119 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat , By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat , -
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat liver extract, rat spleen extract, rat brain extract, rat cardiac muscle extract, mouse liver extract, mouse spleen extract, mouse brain extract, mouse kidney extract, mouse thymus extract, mouse lung extract, HEPA1-6 whole cell, NIH3T3 whole cell
IHC: Rat Spleen Tissue, mouse intestine tissue
Validation Images & Assay Conditions
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Anti-Fas antibody, PA1119, Western blotting
Lane 1: Recombinant Mouse FAS Protein 10ng
Lane 2: Recombinant Mouse FAS Protein 5ng
Lane 3: Recombinant Mouse FAS Protein 2.5ng
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Anti-Fas antibody, PA1119, IHC(P)
IHC(P): Rat Spleen Tissue Lysate
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Western blot analysis of FAS expression in rat liver extract (lane 1), rat spleen extract (lane 2), rat brain extract (lane 3) and rat cardiac muscle extract (lane 4). FAS at 50KD was detected using rabbit anti-FAS Antigen Affinity purified polyclonal antibody (Catalog # PA1119) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
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Western blot analysis of FAS expression in mouse liver extract (lane 1), mouse spleen extract (lane 2), mouse brain extract (lane 3) mouse kidney extract (lane 4), mouse thymus extract (lane 5), mouse lung extract (lane 6), HEPA1-6 whole cell lysates (lane 7) and NIH3T3 whole cell lysates (lane 8). FAS at 50KD was detected using rabbit anti-FAS Antigen Affinity purified polyclonal antibody (Catalog # PA1119) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
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Figure 5. IHC analysis of FAS using anti-FAS antibody (PA1119).
FAS was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FAS Antibody (PA1119) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Fas (Source: Uniprot.org, NCBI)
Gene Name
Fas
Full Name
Tumor necrosis factor receptor superfamily member 6
Weight
36835 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 6;Apo-1 antigen;Apoptosis-mediating surface antigen FAS;FASLG receptor;CD95;Fas;Apt1, Tnfrsf6; FAS ALPS1A, APO-1, APT1, CD951, FASTM, TNFRSF6, FAS Fas cell surface death receptor tumor necrosis factor receptor superfamily member 6|APO-1 cell surface |CD95 |FASLG receptor|Fas (TNF receptor superfamily, member 6)|Fas AMA|TNF receptor superfamily member 6|apoptosis 1|apoptosis signaling receptor FAS|apoptosis-mediating surface FAS|mutant tumor necrosis receptor superfamily member 6|tumor necrosis factor receptor superfamily, member 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Fas, check out the Fas Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Fas: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fas Antibody Picoband® (PA1119)
Hello CJ!
PA1119 has been cited in 21 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lanfen Chen,Wei Chen,Mengbei Zhang et al.Comparison of therapeutic effects of melatonin by two different routes in focal cerebral ischemic rats.Journal of Neurorestoratology 2019,07(01):47-53.
Species: Rat
PA1119 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Yan Zhang,Linchao Zhang,JiaLu Bao et al.Perfluorooctanoic Acid Exposure in Early Pregnancy Induces Oxidative Stress in The Uterus and Liver in Mice,13 February 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-160447
Species: Mouse
PA1119 usage in article: APP:IHC, SAMPLE:UTREUS, DILUTION:NA
Osteoprotegerin Induces Apoptosis of Osteoclasts and Osteoclast Precursor Cells via the Fas/Fas Ligand Pathway
HSP70 inhibits stress-induced cardiomyocyte apoptosis by competitively binding to FAF1
Construction and identification of Fas-targeting siRNA-expressing plasmid
Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells
Significance of Fas and FasL protein expression in cardiac carcinoma and local lymph node tissues
Yeung CK, Wang G, Yao Y, Liang J, Tenny Chung CY, Chuai M, Lee KK, Yang X. Cell Death Dis. 2017 Mar 23;8(3):e2697. doi: 10.1038/cddis.2017.91. BRE modulates granulosa cell death to affect ovarian follicle development and atresia in the mouse
Weng T, Xie Y, Yi L, Huang J, Luo F, Du X, Chen L, Liu C, Chen D, Chen L. Osteoarthritis Cartilage. 2014 Aug;22(8):1197-205. Doi: 10.1016/J.Joca.2014.06.031. Epub 2014 Jul 4. Loss Of Vhl In Cartilage Accelerated The Progression Of Age-Associated A...
Li W, Xia D, Wang Y, Li Y, Xue Y, Wu X, Ye Z. J Huazhong Univ Sci Technolog Med Sci. 2011 Dec;31(6):794-8. Doi: 10.1007/S11596-011-0679-6. Epub 2011 Dec 16. Relationship Between Aberrant Methylation Of Fas Promoter And Biological Behavior Of Bladd...
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-Fas Antibody Picoband®
Question
Does anti-Fas antibody PA1119 work for IHC with right ovary?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
According to the expression profile of right ovary, FAS is highly expressed in right ovary. So, it is likely that anti-Fas antibody PA1119 will work for IHC with right ovary.
Boster Scientific Support
Answered: 2020-01-10
Question
We need to test anti-Fas antibody PA1119 on rat right ovary for research purposes, then I may be interested in using anti-Fas antibody PA1119 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The products we sell, including anti-Fas antibody PA1119, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-13
Question
I was wanting to use your anti-Fas antibody for IHC for rat right ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat right ovary identification?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
You can see on the product datasheet, PA1119 anti-Fas antibody has been validated for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat right ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-06
Question
I am interested in using your anti-Fas antibody for negative regulation of schwann cell migration studies. Has this antibody been tested with western blotting on mouse intestine tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
Thank you for your inquiry. This PA1119 anti-Fas antibody is validated on mouse intestine tissue, rat spleen tissue, nih3t3 whole cell lysates, tissue lysate. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-09
Question
My question regarding product PA1119, anti-Fas antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1119 anti-Fas antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-20
Question
Here is the WB image, lot number and protocol we used for right ovary using anti-Fas antibody PA1119. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-09
Question
Would anti-Fas antibody PA1119 work on horse IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
Our lab technicians have not validated anti-Fas antibody PA1119 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Fas antibody PA1119 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-02
Question
Is a blocking peptide available for product anti-Fas antibody (PA1119)?
C. Jha
Verified customer
Asked: 2019-08-22
Answer
We do provide the blocking peptide for product anti-Fas antibody (PA1119). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-22
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-Fas antibody PA1119. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-24
Question
Would PA1119 anti-Fas antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
You can see on the product datasheet, PA1119 anti-Fas antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-24
Question
I see that the anti-Fas antibody PA1119 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-10
Question
We bought anti-Fas antibody for WB on peripheral blood lymphocyte last year. I am using rat, and We are going to use the antibody for IHC next. I was wanting to use examining peripheral blood lymphocyte as well as urinary bladder in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
I have checked the website and datasheets of our anti-Fas antibody and it appears that PA1119 has been tested on rat in both WB and IHC. Thus PA1119 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-05-03
Question
Do you have a BSA free version of anti-Fas antibody PA1119 available?
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Fas antibody PA1119 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-20
Question
Our lab were satisfied with the WB result of your anti-Fas antibody. However we have seen positive staining in cervix carcinoma isoform 2: secreted. using this antibody. Is that expected? Could you tell me where is FAS supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
From literature, cervix carcinoma does express FAS. Generally FAS expresses in isoform 1: cell membrane, isoform 2: secreted., isoform 3: secreted., isoform 4: secreted., isoform 5: secreted., isoform 6: secreted. Regarding which tissues have FAS expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Liver, Pubmed ID: 19159218, 24275569
Peripheral blood lymphocyte, Pubmed ID: 14702039
Urinary bladder, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-07-21
Question
We are currently using anti-Fas antibody PA1119 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on dog tissues as well?
P. Taylor
Verified customer
Asked: 2017-01-17
Answer
The anti-Fas antibody (PA1119) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-01-17
Question
We have been able to see staining in rat cervix carcinoma. Any tips? Is anti-Fas antibody supposed to stain cervix carcinoma positively?
J. Bhatt
Verified customer
Asked: 2014-10-23
Answer
Based on literature cervix carcinoma does express FAS. Based on Uniprot.org, FAS is expressed in right ovary, peripheral blood lymphocyte, urinary bladder, cervix carcinoma, liver, among other tissues. Regarding which tissues have FAS expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Liver, Pubmed ID: 19159218, 24275569
Peripheral blood lymphocyte, Pubmed ID: 14702039
Urinary bladder, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-10-23
Question
Is this PA1119 anti-Fas antibody reactive to the isotypes of FAS?
D. Anderson
Verified customer
Asked: 2014-10-23
Answer
The immunogen of PA1119 anti-Fas antibody is A synthetic peptide corresponding to a sequence at the N-terminus of rat Fas (87-109aa, YTDRKHYSDKCRRCAFCDEGHGL). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-10-23
