Click image to see more details
Product Info Summary
| SKU: | A03662-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Fanca/FAA Antibody Picoband®
View all FACA/FANCA Antibodies
SKU/Catalog Number
A03662-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fanca/FAA Antibody Picoband® catalog # A03662-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Fanca/FAA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03662-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Fanca/FAA recombinant protein (Position: E1272-F1439).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03662-2 is reactive to Fanca in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
180 kDa
Calculated molecular weight
28461 MW
Background of FACA/FANCA
Fanconi anaemia, complementation group A, also known as FAA, FACA and FANCA, is a protein which in humans is encoded by the FANCA gene. Acts upstream of or within several processes, including gonad development; regulation of CD40 signaling pathway; and regulation of regulatory T cell differentiation. Predicted to be located in nucleoplasm. Predicted to be part of Fanconi anaemia nuclear complex. Is expressed in several structures, including gut; liver; lung; reproductive system; and spleen. Used to study Fanconi anemia complementation group A. Human ortholog(s) of this gene implicated in several diseases, including Fanconi anemia (multiple); acute myeloid leukemia; esophagus squamous cell carcinoma; ovarian disease (multiple); and skin melanoma. Orthologous to human FANCA (FA complementation group A).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03662-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat testis tissue, mouse thymus tissue
FCM: ANA-1 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Fanca/FAA using anti-Fanca/FAA antibody (A03662-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fanca/FAA antigen affinity purified polyclonal antibody (Catalog # A03662-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fanca/FAA at approximately 180 kDa. The expected band size for Fanca/FAA is at 161 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of ANA-1 cells using anti-Fanca/FAA antibody (A03662-2).
Overlay histogram showing ANA-1 cells stained with A03662-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fanca/FAA Antibody (A03662-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Fanca (Source: Uniprot.org, NCBI)
Gene Name
Fanca
Full Name
Fanconi anemia group A protein
Weight
28461 MW
Alternative Names
DNA replication licensing factor MCM5; CDC46 homolog; P1-CDC46; MCM5; CDC46 FANCA FA, FA-H, FA1, FAA, FACA, FAH, FANCH FA complementation group A Fanconi anemia group A protein|Fanconi anemia complementation group A|Fanconi anemia, complementation group H|Fanconi anemia, type 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Fanca, check out the Fanca Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Fanca: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fanca/FAA Antibody Picoband® (A03662-2)
Hello CJ!
No publications found for A03662-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Fanca/FAA Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Fanca/FAA Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
