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Product Info Summary
| SKU: | EK0474 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Rat Neurotrophin-3 ELISA Kit PicoKine®
SKU/Catalog Number
EK0474
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Neurotrophin-3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Ntf3 in cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Neurotrophin-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0474)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Y139-T257
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0474 for more details
Cross-reactivity
There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
Reactive Species
EK0474 is reactive to Ntf3 in Rat samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0474 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of NT-3
Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0474-CAP | Anti-Rat Ntf3 Pre-coated 96-well strip microplate | 1 |
| EK0474-ST | Rat Ntf3 Standard | 2 vials, 10 ng/tube |
| EK0474-DA | Rat Ntf3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.003 | 0.056 | 0.097 | 0.203 | 0.388 | 0.691 | 1.286 | 1.906 |
Data Example Images
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Rat Neurotrophin-3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect NT-3, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 15.6 pg/ml..
Some articles we found to cite concentrations of NT-3 in samples: 23074106 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Neurotrophin-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 41 | 173 | 464 | 41 | 177 | 504 |
| Standard deviation | 2.25 | 12.45 | 23.66 | 2.99 | 15.53 | 27.72 |
| CV (%) | 5.5% | 7.2% | 5.5% | 7.3% | 9% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of NT-3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 41 | 38 | 39 | 39 | 39 | 1.08 | 2.7% |
| Sample 2 | 173 | 159 | 176 | 168 | 169 | 6.44 | 3.8% |
| Sample 3 | 464 | 416 | 419 | 479 | 444 | 27.53 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For Ntf3 (Source: Uniprot.Org, NCBI)
Gene Name
Ntf3
Full Name
Neurotrophin-3
Weight
29645 MW
Superfamily
NGF-beta family
Alternative Names
Neurotrophin-3;NT-3;HDNF;Nerve growth factor 2;NGF-2;Neurotrophic factor;Ntf3;Ntf-3; NTF3 HDNF, NGF-2, NGF2, NT-3, NT3 neurotrophin 3 neurotrophin-3|nerve growth factor 2|neurotrophic factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Ntf3, check out the Ntf3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ntf3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Neurotrophin-3 ELISA Kit PicoKine® (EK0474)
Hello CJ!
EK0474 has been cited in 18 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats.
Electroacupuncture Promotes the Differentiation of Transplanted Bone Marrow Mesenchymal Stem Cells Overexpressing TrkC into Neuron-Like Cells in Transected Spinal Cord of Rats:
Triptolide improves neurobehavioral functions, inflammation, and oxidative stress in rats under deep hypothermic circulatory arrest
Swimming Training Reduces Neuroma Pain by Regulating Neurotrophins
Governor Vessel Electro-Acupuncture Promotes the Intrinsic Growth Ability of Spinal Neurons through Activating Calcitonin Gene-Related Peptide/α-Calcium/Calmodulin-Dependent Protein Kinase/Neurotrophin-3 Pathway after Spinal Cord Injury
Combination of Electroacupuncture and Grafted Mesenchymal Stem Cells Overexpressing TrkC Improves Remyelination and Function in Demyelinated Spinal Cord of Rats
Combination of adenoviral vector-mediated neurotrophin-3 gene transfer and retinoic acid promotes adult bone marrow cells to differentiate into neuronal phenotypes
Cochlear nucleus whole mount explants promote the differentiation of neuronal stem cells from the cochlear nucleus in co-culture experiments
An NT-3-releasing bioscaffold supports the formation of TrkC-modified neural stem cell-derived neural network tissue with efficacy in repairing spinal cord injury
Low-intensity pulsed ultrasound regulates proliferation and differentiation of neural stem cells through notch signaling pathway
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Customer Q&As
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9 Customer Q&As for Rat Neurotrophin-3 ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I have obtained NT-3 level in serum.
B. Hall
Verified customer
Asked: 2020-12-03
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-12-03
Question
Q: if the enzyme conjugated NT-3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the NT-3 antigen?
K. Martinez
Verified customer
Asked: 2020-11-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-05
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
K. Singh
Verified customer
Asked: 2019-12-19
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-19
Question
Are the baculovirus expressing systems used for manufacturing the kits? Keyword: expression system
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
sf21 was the expression system for both the standard and recombinant protein immunogen for NT3 ELISA kit.
Boster Scientific Support
Answered: 2019-04-11
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
M. Collins
Verified customer
Asked: 2018-11-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-11-25
Question
Q: What is the optimal O.D. value for NT-3 ELISA kit? I used your NT-3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain NT-3 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this NT-3 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of NT-3 that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2017-10-13
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring NT-3 using the EK0474 Rat NT-3 Picokine® ELISA Kit?
B. Varma
Verified customer
Asked: 2017-08-03
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2017-08-03
Question
Q: how can I thaw whole blood sample for NT-3 ELISA after freezing?
J. Smith
Verified customer
Asked: 2017-04-21
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test NT-3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-04-21
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of NT-3 in Rat serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
R. Krishna
Verified customer
Asked: 2014-07-07
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the NT-3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2014-07-07
