Product Info Summary
SKU: | A07467 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-F1A alpha FEM1B Antibody
SKU/Catalog Number
A07467
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-F1A alpha FEM1B Antibody (Catalog # A07467). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
F1A alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-F1A alpha FEM1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07467)
Host
Rabbit
Contents
F1A alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
F1A antibody was raised against a 14 amino acid peptide near the carboxy terminus of human F1A. The immunogen is located within the last 50 amino acids of F1A alpha.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PHAP III has no cross-reaction to PHAP I and PHAP I2a.
Reactive Species
A07467 is reactive to FEM1B in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
70264 MW
Background of FEM1B
Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aalpha and FEM1β. F1Aalpha/FEM1β is the homologue of C. elegans sex determining protein FEM-1. FEM-1/F1Aalpha is cleaved by CED-3 and caspase. FEM-1/F1Aalpha associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aalpha induces apoptosis. F1Aalpha is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aalpha is expressed in a variety of human and mouse tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07467 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
F1Ab antibody can be used for detection of F1Ab by Western blot at 1 μg/mL. An approximately 70 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 μg/mL.
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Immunohistochemistry of F1Aα in mouse liver tissue with F1Aα antibody at 5 μg/mL.
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Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 μg/mL.
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Immunofluorescence of F1A alpha in mouse liver tissue with F1A alpha antibody at 20 μg/ml.
Green: F1A alpha Antibody (A07467)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For FEM1B (Source: Uniprot.org, NCBI)
Gene Name
FEM1B
Full Name
Protein fem-1 homolog B
Weight
70264 MW
Superfamily
fem-1 family
Alternative Names
F1AA, F1A-ALPHA, FEM1-beta, F1AA, KIAA0396, Protein fem-1 homolog B, FEM1b FEM1B F1A-ALPHA, F1AA, FEM1-beta fem-1 homolog B protein fem-1 homolog B|FEM-1-like death receptor binding protein|fem-1-like death receptor-binding protein alpha|fem-1-like in apoptotic pathway protein alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FEM1B, check out the FEM1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FEM1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-F1A alpha FEM1B Antibody (A07467)
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