Anti-Ezrin (phospho-Y353) Antibody

Product Name

Anti-Ezrin (phospho-Y353) Antibody

View all Ezrin Antibodies

SKU/Catalog Number

A01750Y353

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Ezrin (phospho-Y353) Antibody catalog # A01750Y353. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ezrin (phospho-Y353) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01750Y353)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Ezrin around the phosphorylation site of Y146.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01750Y353 is reactive to EZR in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

Calculated molecular weight

69413 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01750Y353 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000

Positive Control

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-Ezrin (phospho-Y353) Antibody A01750Y353

Western blot (WB) analysis of p-Ezrin (Y353) pAb at 1:500 dilution
Lane1:A549 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:PMVEC whole cell lysate(40ug)
Lane4:3T3-L1 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For EZR (Source: Uniprot.org, NCBI)

Alternative Names

CVIL; CVL; Cytovillin 2; Cytovillin; DKFZp762H157; EZR; Ezrin; FLJ26216; p81; VIL2; VIL2MGC1584; villin 2 (ezrin); Villin 2; villin-2 EZR CVIL, CVL, HEL-S-105, VIL2 ezrin ezrin|cytovillin 2|epididymis secretory protein Li 105|p81|villin 2 (ezrin)

For more info on EZR, check out the EZR Infographic

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In this infographic, you will see the following information for EZR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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