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Product Info Summary
| SKU: | M02812 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-ERK5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02812
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ERK5 Rabbit Monoclonal Antibody catalog # M02812. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERK5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02812)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HCD-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ERK5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02812 is reactive to MAPK7 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120 kDa
Calculated molecular weight
88386 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02812 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of ERK5 expression in Hela cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MAPK7 (Source: Uniprot.org, NCBI)
Gene Name
MAPK7
Full Name
Mitogen-activated protein kinase 7
Weight
88386 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase 7;MAP kinase 7;MAPK 7;2.7.11.24;Big MAP kinase 1;BMK-1;Extracellular signal-regulated kinase 5;ERK-5;MAPK7;BMK1, ERK5, PRKM7; MAPK7 BMK1, ERK4, ERK5, PRKM7 mitogen-activated protein kinase 7 mitogen-activated protein kinase 7|BMK-1|BMK1 kinase|ERK-5|MAP kinase 7|MAPK 7|big MAP kinase 1|extracellular-signal-regulated kinase 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPK7, check out the MAPK7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERK5 Rabbit Monoclonal Antibody (M02812)
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No publications found for M02812
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1 Customer Q&As for Anti-ERK5 Rabbit Monoclonal Antibody
Question
We are currently using anti-ERK5 Rabbit Monoclonal antibody M02812 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
A. Rodriguez
Verified customer
Asked: 2014-08-01
Answer
The anti-ERK5 Rabbit Monoclonal antibody (M02812) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-08-01
