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Product Info Summary
| SKU: | M00104 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ERK1 MAPK3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00104
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ERK1 MAPK3 Rabbit Monoclonal Antibody catalog # M00104. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERK1 MAPK3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00104)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADF-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ERK1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00104 is reactive to MAPK3 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
31 kDa, 28 kDa, 17 kDa
Calculated molecular weight
43136 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00104 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:250
IP 1:50
FC 1:50
Positive Control
IHC: human breast carcinoma tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Western blot analysis of ERK1 in NIH-3T3 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ERK1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MAPK3 (Source: Uniprot.org, NCBI)
Gene Name
MAPK3
Full Name
Mitogen-activated protein kinase 3
Weight
43136 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;2.7.11.24;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;Microtubule-associated protein 2 kinase;p44-ERK1;MAPK3;ERK1, PRKM3; MAPK3 ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK mitogen-activated protein kinase 3 mitogen-activated protein kinase 3|MAPK 1|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1|insulin-stimulated MAP2 kinase|microtubule-associated protein 2 kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPK3, check out the MAPK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERK1 MAPK3 Rabbit Monoclonal Antibody (M00104)
Hello CJ!
M00104 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang C,Wang J,Liu X,Han Z,Aimin Jiang,Wei Z,Yang Z.Cl-amidine attenuates lipopolysaccharide-induced mouse mastitis by inhibiting NF-κB, MAPK, NLRP3 signaling pathway and neutrophils extracellular traps release.Microb Pathog.2020 Sep 24;149:104530.doi:10.1
Species: Mouse
M00104 usage in article: APP:WB, SAMPLE:MAMMARY TISSUE, DILUTION:1:1000
Synthesis and biological evaluation of novel N, N%u2032-disubstituted urea and thiourea derivatives as potential anti-melanoma agents
Disulfiram inhibits TGF-?-induced epithelial-mesenchymal transition and stem-like features in breast cancer via ERK/NF-?B/Snail pathway
Fructus phyllanthi tannin fraction induces apoptosis and inhibits migration and invasion of human lung squamous carcinoma cells in vitro via MAPK/MMP pathways
Ma Hr, Wang J, Chen Yf, Chen H, Wang Ws, Aisa Ha. Int J Mol Med. 2014 Jun;33(6):1627-34. Doi: 10.3892/Ijmm.2014.1722. Epub 2014 Apr 3. Icariin And Icaritin Stimulate The Proliferation Of Skbr3 Cells Through The Gper1-Mediated Modulation Of The Egf...
Hu Cp, Feng Jt, Tang Yl, Zhu Jq, Lin Mj, Yu Me. Mediators Inflamm. 2006;2006(5):84829. Lif Upregulates Expression Of Nk-1R In Nhbe Cells.
Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-ERK1 MAPK3 Rabbit Monoclonal Antibody
Question
Our lab used your anti-ERK1 Rabbit Monoclonal antibody for IF on lymph last year. I am using mouse, and We are going to use the antibody for WB next. My lab would like examining lymph as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
I viewed the website and datasheets of our anti-ERK1 Rabbit Monoclonal antibody and it seems that M00104 has been validated on mouse in both IF and WB. Thus M00104 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-08
Question
Would M00104 anti-ERK1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
It shows on the product datasheet, M00104 anti-ERK1 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-31
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hepatoma using anti-ERK1 Rabbit Monoclonal antibody M00104. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-24
Question
My team were content with the WB result of your anti-ERK1 Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma caveola using this antibody. Is that expected? Could you tell me where is MAPK3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
Based on literature, cervix carcinoma does express MAPK3. Generally MAPK3 expresses in cytoplasm. nucleus. membrane, caveola. Regarding which tissues have MAPK3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hepatoma, Pubmed ID: 1540184, 7687743
Leukemic T-cell, Pubmed ID: 19690332
Lymph, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-13
Question
Will anti-ERK1 Rabbit Monoclonal antibody M00104 work for IF with hepatoma?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
According to the expression profile of hepatoma, MAPK3 is highly expressed in hepatoma. So, it is likely that anti-ERK1 Rabbit Monoclonal antibody M00104 will work for IF with hepatoma.
Boster Scientific Support
Answered: 2019-10-24
Question
Please see the WB image, lot number and protocol we used for hepatoma using anti-ERK1 Rabbit Monoclonal antibody M00104. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-02
Question
We are currently using anti-ERK1 Rabbit Monoclonal antibody M00104 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The anti-ERK1 Rabbit Monoclonal antibody (M00104) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-30
Question
Is this M00104 anti-ERK1 Rabbit Monoclonal antibody reactive to the isotypes of MAPK3?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The immunogen of M00104 anti-ERK1 Rabbit Monoclonal antibody is A synthesized peptide derived from human ERK1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-14
Question
you antibody to test anti-ERK1 Rabbit Monoclonal antibody M00104 on human hepatoma for research purposes, then I may be interested in using anti-ERK1 Rabbit Monoclonal antibody M00104 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
The products we sell, including anti-ERK1 Rabbit Monoclonal antibody M00104, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-12
Question
I have a question about product M00104, anti-ERK1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-04-29
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00104 anti-ERK1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-29
Question
I was wanting to use your anti-ERK1 Rabbit Monoclonal antibody for IF for human hepatoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hepatoma identification?
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
It shows on the product datasheet, M00104 anti-ERK1 Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human hepatoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-15
Question
Do you have a BSA free version of anti-ERK1 Rabbit Monoclonal antibody M00104 available?
J. Gonzalez
Verified customer
Asked: 2018-01-01
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ERK1 Rabbit Monoclonal antibody M00104 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-01
Question
Is a blocking peptide available for product anti-ERK1 Rabbit Monoclonal antibody (M00104)?
H. Krishna
Verified customer
Asked: 2015-06-05
Answer
We do provide the blocking peptide for product anti-ERK1 Rabbit Monoclonal antibody (M00104). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-06-05
Question
I see that the anti-ERK1 Rabbit Monoclonal antibody M00104 works with IF, what is the protocol used to produce the result images on the product page?
R. Carter
Verified customer
Asked: 2014-10-31
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-10-31
Question
We have seen staining in human cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-ERK1 Rabbit Monoclonal antibody supposed to stain cervix carcinoma erythroleukemia positively?
D. Johnson
Verified customer
Asked: 2014-06-18
Answer
From literature cervix carcinoma erythroleukemia does express MAPK3. From Uniprot.org, MAPK3 is expressed in right frontal lobe, hepatoma, lymph, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MAPK3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hepatoma, Pubmed ID: 1540184, 7687743
Leukemic T-cell, Pubmed ID: 19690332
Lymph, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-06-18
