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Product Info Summary
| SKU: | M00388 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ERCC1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00388
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ERCC1 Rabbit Monoclonal Antibody catalog # M00388. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERCC1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00388)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOFA-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ERCC1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00388 is reactive to ERCC1 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
125 kDa
Calculated molecular weight
32562 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00388 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of ERCC1 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ERCC1 (Source: Uniprot.org, NCBI)
Gene Name
ERCC1
Full Name
DNA excision repair protein ERCC-1
Weight
32562 MW
Superfamily
ERCC1/RAD10/SWI10 family
Alternative Names
DNA excision repair protein ERCC-1;ERCC1; ERCC1 COFS4, RAD10, UV20 ERCC excision repair 1, endonuclease non-catalytic subunit DNA excision repair protein ERCC-1|excision repair cross-complementation group 1|excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERCC1, check out the ERCC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERCC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERCC1 Rabbit Monoclonal Antibody (M00388)
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No publications found for M00388
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Customer Q&As
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6 Customer Q&As for Anti-ERCC1 Rabbit Monoclonal Antibody
Question
See below the WB image, lot number and protocol we used for lung uterus using anti-ERCC1 Rabbit Monoclonal antibody M00388. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-27
Question
I was wanting to use your anti-ERCC1 Rabbit Monoclonal antibody for IF for human lung uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung uterus identification?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
As indicated on the product datasheet, M00388 anti-ERCC1 Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human lung uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-09
Question
Would anti-ERCC1 Rabbit Monoclonal antibody M00388 work on monkey WB with lung uterus?
A. Mitchell
Verified customer
Asked: 2018-12-26
Answer
Our lab technicians have not tested anti-ERCC1 Rabbit Monoclonal antibody M00388 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-ERCC1 Rabbit Monoclonal antibody M00388 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey lung uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung uterus using anti-ERCC1 Rabbit Monoclonal antibody M00388. Let me know if you need anything else.
S. Carter
Verified customer
Asked: 2017-03-17
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-17
Question
Will anti-ERCC1 Rabbit Monoclonal antibody M00388 work for IF with lung uterus?
P. Wu
Verified customer
Asked: 2016-08-24
Answer
According to the expression profile of lung uterus, ERCC1 is highly expressed in lung uterus. So, it is likely that anti-ERCC1 Rabbit Monoclonal antibody M00388 will work for IF with lung uterus.
Boster Scientific Support
Answered: 2016-08-24
Question
Can you help my question with product M00388, anti-ERCC1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Kulkarni
Verified customer
Asked: 2015-10-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00388 anti-ERCC1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-10-02
