Product Info Summary
SKU: | M00388-2 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Mouse |
Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-ERCC1 Mouse Monoclonal Antibody [Clone ID: OTI1C10]
SKU/Catalog Number
M00388-2
Size
100 µl
Description
Boster Bio ERCC1 mouse monoclonal antibody, clone OTI1C10 (formerly 1C10). Catalog# M00388-2. Tested in FC, IF, WB. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ERCC1 Mouse Monoclonal Antibody [Clone ID: OTI1C10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00388-2)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1C10
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human ERCC1 (NP_001974) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00388-2 is reactive to ERCC1 in Human, Mouse
Reconstitution
Calculated molecular weight
32.562kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00388-2 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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HEK293T cells transfected with either ERCC1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERCC1 antibody (M00388-2)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-ERCC1 mouse monoclonal antibody (M00388-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.
Protein Target Info & Infographic
Gene/Protein Information For ERCC1 (Source: Uniprot.org, NCBI)
Gene Name
ERCC1
Full Name
DNA excision repair protein ERCC-1
Weight
32.562kDa
Superfamily
ERCC1/RAD10/SWI10 family
Alternative Names
COFS4; DNA excision repair protein ERCC-1; ERCC1; excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence); RAD10; UV20 ERCC1 COFS4, RAD10, UV20 ERCC excision repair 1, endonuclease non-catalytic subunit DNA excision repair protein ERCC-1|excision repair cross-complementation group 1|excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERCC1, check out the ERCC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERCC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERCC1 Mouse Monoclonal Antibody [Clone ID: OTI1C10] (M00388-2)
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