Click image to see more details
Product Info Summary
| SKU: | A01681-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-EPS15 Antibody Picoband®
SKU/Catalog Number
A01681-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EPS15 Antibody Picoband® catalog # A01681-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EPS15 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01681-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human EPS15 recombinant protein (Position: M1-K295).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01681-1 is reactive to EPS15 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
138 kDa
Calculated molecular weight
50514 MW
Background of EPS15
Epidermal growth factor receptor substrate 15 is a protein that in humans is encoded by the EPS15 gene. It is mapped to 1p32.3. This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01681-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue, mouse NIH-3T3 whole cell, human Hela whole cell, human Jurkat whole cell, human HEK293 whole cell, human PC-3 whole cell, human THP-1 whole cell
FCM: A431 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of EPS15 using anti-EPS15 antibody (A01681-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: mouse NIH-3T3 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: human HEK293 whole cell lysates,
Lane 7: human PC-3 whole cell lysates,
Lane 8: human THP-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPS15 antigen affinity purified polyclonal antibody (Catalog # A01681-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPS15 at approximately 138KD. The expected band size for EPS15 is at 138KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of A431 cells using anti-EPS15 antibody (A01681-1).
Overlay histogram showing A431 cells stained with A01681-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPS15 Antibody (A01681-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For EPS15 (Source: Uniprot.org, NCBI)
Gene Name
EPS15
Full Name
Epidermal growth factor receptor substrate 15
Weight
50514 MW
Alternative Names
Epidermal growth factor receptor substrate 15; Protein Eps15; Protein AF-1p; EPS15; AF1P EPS15 AF-1P, AF1P, MLLT5 epidermal growth factor receptor pathway substrate 15 epidermal growth factor receptor substrate 15|ALL1 fused gene from chromosome 1|protein AF-1p
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPS15, check out the EPS15 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPS15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EPS15 Antibody Picoband® (A01681-1)
Hello CJ!
No publications found for A01681-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-EPS15 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-EPS15 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
