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Product Info Summary
| SKU: | A10011-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Epigen/Epgn Antibody Picoband®
SKU/Catalog Number
A10011-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Epigen/Epgn Antibody Picoband® catalog # A10011-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Epigen/Epgn Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10011-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Epigen recombinant protein (Position: A19-K109). Mouse Epigen shares 82.8% amino acid (aa) sequence identity with human Epigen.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10011-2 is reactive to Epgn in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
16799 MW
Background of EPGN
Epigen also known as epithelial mitogen is a protein that in humans is encoded by the EPGN gene. The protein encoded by this gene is a member of the epidermal growth factor family. Members of this family are ligands for the epidermal growth factor receptor and play a role in cell survival, proliferation and migration. This protein has been reported to have high mitogenic activity but low affinity for its receptor. Expression of this transcript and protein have been reported in cancer specimens of the breast, bladder, and prostate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10011-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry, 2-5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
IHC: mouse testis tissue, rat testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Epigen using anti-Epigen antibody (A10011-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epigen antigen affinity purified polyclonal antibody (Catalog # A10011-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epigen at approximately 17 kDa. The expected band size for Epigen is at 17 kDa.
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Figure 2. IHC analysis of Epigen using anti-Epigen antibody (A10011-2).
Epigen was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Epigen Antibody (A10011-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of Epigen using anti-Epigen antibody (A10011-2).
Epigen was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Epigen Antibody (A10011-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Epgn (Source: Uniprot.org, NCBI)
Gene Name
Epgn
Full Name
Epigen
Weight
16799 MW
Alternative Names
Epigen;Epithelial mitogen;EPG;Epgn; Epgn|2310069M11Rik, epi, epigen|epithelial mitogen|epigen|EPG
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Epgn, check out the Epgn Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Epgn: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Epigen/Epgn Antibody Picoband® (A10011-2)
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Customer Q&As
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7 Customer Q&As for Anti-Epigen/Epgn Antibody Picoband®
Question
Is this A10011-2 anti-Epigen/Epgn antibody reactive to the isotypes of EPGN?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The immunogen of A10011-2 anti-Epigen/Epgn antibody is E. coli-derived mouse Epigen recombinant protein (Position: A19-K109). Mouse Epigen shares 82.8% amino acid (aa) sequence identity with human Epigen. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-09
Question
I see that the anti-Epigen/Epgn antibody A10011-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-11
Question
My question regarding product A10011-2, anti-Epigen/Epgn antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10011-2 anti-Epigen/Epgn antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-09
Question
Would anti-Epigen/Epgn antibody A10011-2 work for WB with esophagus mucosa?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
According to the expression profile of esophagus mucosa, EPGN is highly expressed in esophagus mucosa. So, it is likely that anti-Epigen/Epgn antibody A10011-2 will work for WB with esophagus mucosa.
Boster Scientific Support
Answered: 2020-01-10
Question
Would A10011-2 anti-Epigen/Epgn antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
It shows on the product datasheet, A10011-2 anti-Epigen/Epgn antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-21
Question
Here is the WB image, lot number and protocol we used for esophagus mucosa using anti-Epigen/Epgn antibody A10011-2. Please let me know if you require anything else.
B. Taylor
Verified customer
Asked: 2017-10-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-13
Question
We are currently using anti-Epigen/Epgn antibody A10011-2 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
K. Miller
Verified customer
Asked: 2013-09-25
Answer
The anti-Epigen/Epgn antibody (A10011-2) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-09-25
