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Product Info Summary
| SKU: | A10011-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Epigen/EPGN Antibody Picoband®
SKU/Catalog Number
A10011-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Epigen/EPGN Antibody Picoband® catalog # A10011-1. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Epigen/EPGN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10011-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Epigen recombinant protein (Position: A23-K110). Human Epigen shares 85.7% amino acid (aa) sequence identity with mouse Epigen.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10011-1 is reactive to EPGN in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
17091 MW
Background of EPGN
Epigen, also known as epithelial mitogen, is a protein that in humans is encoded by the EPGN gene. The protein encoded by this gene is a member of the epidermal growth factor family. Members of this family are ligands for the epidermal growth factor receptor and play a role in cell survival, proliferation and migration. This protein has been reported to have high mitogenic activity but low affinity for its receptor. Expression of this transcript and protein have been reported in cancer specimens of the breast, bladder, and prostate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10011-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: human A431 whole Cell, rat PC-12 whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Epigen using anti-Epigen antibody (A10011-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A431 whole Cell lysate,
Lane 2: rat PC-12 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epigen antigen affinity purified polyclonal antibody (Catalog # A10011-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epigen at approximately 17KD. The expected band size for Epigen is at 17KD.
Protein Target Info & Infographic
Gene/Protein Information For EPGN (Source: Uniprot.org, NCBI)
Gene Name
EPGN
Full Name
Epigen
Weight
17091 MW
Alternative Names
Epigen;Epithelial mitogen;EPG;EPGN;UNQ3072/PRO9904; Epgn|2310069M11Rik, epi, epigen|epithelial mitogen|epigen|EPG
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPGN, check out the EPGN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPGN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Epigen/EPGN Antibody Picoband® (A10011-1)
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