Ephrin type-A receptor 4 Antibodies

Ephrin type-A receptor 4 (EPHA4)

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.

Highly promiscuous, it has the special property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands such as EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity.

Gene Information

Human
Gene Name:	EPHA4
Uniprot:	P54764
Entrez:	2043

Family

Belongs to:
protein kinase superfamily

Alternative Names

Cek8; EC 2.7.10; EC 2.7.10.1; EK8; EPH receptor A4; EphA4; EPH-like kinase 8; ephrin type-A receptor 4; Hek8; receptor protein-tyrosine kinase HEK8; SEK; Sek1; TYRO1 protein tyrosine kinase; Tyro1; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase TYRO1

Molecular Weight

Mass (kDA):
109.86 kDA

Genomic Context

Human
Location:	2q36.1
Sequence:	2; NC_000002.12 (221418027..221574202, complement)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite. Cell junction, synapse, postsynaptic density membrane. Early endosome. Cell junction, adherens junction. Clustered upon activation and targeted to early endosome.

Diseases

• Nervousness
• Tissue Adhesions
• Malignant Neoplasms
• Spinal Cord Injuries
• Neoplasms
• Crest Syndrome
• Alzheimer's Disease
• Gliosis
• Epithelial Metaplasia
• Pathologic Neovascularization
• Inflammation
• Neoplasm Metastasis
• Malignant Neoplasm Of Prostate

• Carcinoma
• Injury Of Central Nervous System
• Brain Injuries
• Neurodegenerative Disorders
• Muscular Fasciculation
• Tumor Angiogenesis
• Adenocarcinoma

Interacting Proteins

• EFNA2
• EFNA5
• EFNB2
• EFNB3

Pathways

• Axon Guidance
• Regeneration
• Cell Migration
• Cell Adhesion
• Localization
• Segmentation
• Somitogenesis
• Innervation
• Locomotion
• Pathogenesis
• Cell Proliferation
• Neurogenesis
• Synaptic Transmission

• Transport
• System Development
• Cell Development
• Angiogenesis
• Rna Interference
• Central Nervous System Development
• Brain Development

PTMs

• Phosphorylation
• Cleavage

• Methylation
• Dephosphorylation

• Reduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/EPHA4

Boster Bio - Best Uses Of The EPHA4 Marker

Boster bio offers the best primary antibody for many research applications. These antibodies have high-affinity and are available in a variety formats. Researchers can submit results for special samples or applications. They can also apply for product credits that are available to all scientists around the world. You can read on to learn more information about high-affinity antibodies. These are the top uses of EPHA4 markers.

Boster offers high-affinity primary antibodies

Flow cytometry is a powerful technique that allows researchers to identify proteins using a sample of cells. The technique works by using a specific antibody to detect the proteins. Boster Bio stocks the monoclonal and polyclonal primary antibodies used in this application. These antibodies are highly sensitive and have long been used. Boster Bio is a leader of antibody research, offering high affinity primary antibodies against EPHA4 marker.

The EphA10 antibody supplied by Boster Bio was labeled with pHrodo Red (Thermo Fisher Scientific) and incubated with BT549 cells expressing huEphA10 and LysoTracker green. Images were taken using a confo microscope with time-lapse imaging. The images in the figure were obtained from the same experiment.

In order to produce a monoclonal antibody, scientists first immunize host animals with an antigen. Then they extract antibodies from the resulting serum. The selected antibodies are then subject to further application testing. High-affinity primary antibodies are a valuable part of research. They provide high-affinity, highly specific and low-cross-reactive antibodies that can be used against a variety targets.

EphA10 and EphA10 are important markers for EPHA4 identification. EphA10 is a marker that identifies a protein called ephrin-receptor A10. TNBC and EPhA10 also indicate breast cancer. Researchers can use TNBC or EphA10 antibodies to detect and treat these types of tumors.

The anti-EphA10 antibody could be a target for cancer treatment. This antibody was tested in two TNBC syngeneic models. The results showed that this antibody could be a useful candidate for further development. This research could lead to new and exciting treatment options for patients with TNBC. For patients with cancer, a high-affinity antibody to EphA10 might be the best choice.

EphA10 is also known as Ephrin-receptor A10. It is found within the extracellular domain of cells. It is used in immunohistochemistry, immunofluorescence, and cytometry. It is a key marker in the tumor microenvironment. These antibodies can detect EphA10 expression in cell culture.

Dual-labeling allows researchers to ask more specific questions about specimens using two different antibodies. This allows for more context data and more precise answers. With dual labeling, researchers can easily determine which protein is expressed in which cell type. Flow cytometry can then be used to determine the cell type and their composition. Even if the primary antibodies is monoclonal, secondary antibodies can detect a specific type of protein.

Researchers found that siRNA-mediated silencing reduced Akt signaling by sox4 knockdowns in HCC1954 cells. These inhibitors resulted in a decrease in phosphorylation for pAkt, P70S6K and p4EBP1.