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Product Info Summary
| SKU: | A05497 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-EPHA10 NEIL3 Antibody
SKU/Catalog Number
A05497
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-EPHA10 NEIL3 Antibody (Catalog # A05497). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EPHA10 NEIL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05497)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human NEIL3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05497 is reactive to NEIL3 in Human
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
67769 MW
Background of NEI3
DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.
Takao M., J. Biol. Chem. 277:42205-42213(2002).
Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05497 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from COLO cells, HUVEC cells, HepG2 cells and HeLa cells, using NEIL3 antibody A05497.
Protein Target Info & Infographic
Gene/Protein Information For NEIL3 (Source: Uniprot.org, NCBI)
Gene Name
NEIL3
Full Name
Endonuclease 8-like 3
Weight
67769 MW
Superfamily
FPG family
Alternative Names
Endonuclease 8-like 3;3.2.2.-;4.2.99.18;DNA glycosylase FPG2;DNA glycosylase/AP lyase Neil3;Endonuclease VIII-like 3;Nei-like protein 3;NEIL3; NEIL3 FGP2, FPG2, NEI3, ZGRF3, hFPG2, hNEI3 nei like DNA glycosylase 3 endonuclease 8-like 3|DNA glycosylase FPG2|DNA glycosylase hFPG2|DNA glycosylase/AP lyase Neil3|endonuclease VIII-like 3|nei endonuclease VIII-like 3|nei-like protein 3|zinc finger, GRF-type containing 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEIL3, check out the NEIL3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEIL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EPHA10 NEIL3 Antibody (A05497)
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No publications found for A05497
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