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Product Info Summary
| SKU: | M05772 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-EndoPDI Monoclonal Antibody
SKU/Catalog Number
M05772
Size
100ug
Form
liquid
Description
Boster Bio Anti-EndoPDI Monoclonal Antibody catalog # M05772. Tested in ELISA, IP, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EndoPDI Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05772)
Host
Mouse
Contents
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
2E7/7
Isotype
IgG2a
Immunogen
Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the Human EndoPDI protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~48kDa.
Reactive Species
M05772 is reactive to PDI in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
33225 MW
Background of TXNDC5
Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05772 is guaranteed for ELISA, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:200); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EndoPDI Monoclonal Antibody, Clone 2E7/7 (M05772) . Tissue: Cervical Cancer cell line (HeLa) . Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EndoPDI Monoclonal Antibody (M05772) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.
Protein Target Info & Infographic
Gene/Protein Information For PDI (Source: Uniprot.org, NCBI)
Gene Name
PDI
Full Name
Thioredoxin domain-containing protein 5
Weight
33225 MW
Superfamily
protein disulfide isomerase family
Alternative Names
Thioredoxin domain containing 5; ERP46; UNQ364; MGC3178; FLJ21353; FLJ90810; thioredoxin related protein; endothelial protein disulphide isomeras TXNDC5 ENDOPDI, ERP46, HCC-2, HCC2, PDIA15, STRF8, UNQ364 thioredoxin domain containing 5 thioredoxin domain-containing protein 5|ER protein 46|endoplasmic reticulum protein ERp46|endoplasmic reticulum resident protein 46|endothelial protein disulphide isomerase|epididymis secretory sperm binding protein|protein disulfide isomerase family A, member 15|thioredoxin domain containing 5 (endoplasmic reticulum)|thioredoxin related protein|thioredoxin-like protein p46
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDI, check out the PDI Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EndoPDI Monoclonal Antibody (M05772)
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No publications found for M05772
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-EndoPDI Monoclonal Antibody
Question
We are currently using anti-EndoPDI Monoclonal antibody M05772 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
J. Carter
Verified customer
Asked: 2018-06-13
Answer
The anti-EndoPDI Monoclonal antibody (M05772) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-13
Question
Is a blocking peptide available for product anti-EndoPDI Monoclonal antibody (M05772)?
N. Miller
Verified customer
Asked: 2018-01-09
Answer
We do provide the blocking peptide for product anti-EndoPDI Monoclonal antibody (M05772). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-09
Question
Is this M05772 anti-EndoPDI Monoclonal antibody reactive to the isotypes of TXNDC5?
A. Wu
Verified customer
Asked: 2015-11-17
Answer
The immunogen of M05772 anti-EndoPDI Monoclonal antibody is Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-11-17
