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Product Info Summary
| SKU: | A00101-2 |
|---|---|
| Size: | 1mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-IL-1 beta Antibody
View all IL-1 beta/IL-1F2 Antibodies
SKU/Catalog Number
A00101-2
Size
1mg
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-IL-1 beta Antibody (Catalog # A00101-2). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-IL-1 beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00101-2)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
This antibody was prepared by repeated immunizations with recombinant human IL-1ß produced in E.coli. The MW of the recombinant 153 aa IL-1ß was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1ß converting enzyme (ICE, capase-1).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
A00101-2 is reactive to IL1B in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
154537 MW
Background of IL-1 beta/IL-1F2
IL-1 beta (also known as Interleukin-1 beta, IL-1ß and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1ß is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00101-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:500 - 1:2,000
Flow Cytometry: User optimized
IHC: 1:100 - 1:200
IP: 1:400 - 1:800
WB: 1:1,000
Neutralization: 1:100
Validation Images & Assay Conditions
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Figure 1. IHC analysis of IL1B using anti-IL1B antibody (A00101-2).
IL1B was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IL1B Antibody (A00101-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. Western blot analysis of IL1B using anti-IL1B antibody (A00101-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B antigen affinity purified polyclonal antibody (Catalog # A00101-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IL1B.
Click image to see more details
Figure 3. Western blot analysis of IL1B using anti-IL1B antibody (A00101-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B antigen affinity purified polyclonal antibody (Catalog # A00101-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IL1B.
Protein Target Info & Infographic
Gene/Protein Information For IL1B (Source: Uniprot.org, NCBI)
Gene Name
IL1B
Full Name
Interleukin-1 beta
Weight
154537 MW
Superfamily
IL-1 family
Alternative Names
IL-1 beta, interleukin-1 beta, IL-1 beta, Interleukin-1 beta, IL-1ß, catabolin IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL1B, check out the IL1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-1 beta Antibody (A00101-2)
Hello CJ!
A00101-2 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Interleukin-1β of Red nucleus involved in the development of allodynia in spared nerve injury rats
Quantitative proteomic analysis of host responses triggered by Mycobacterium tuberculosis infection in human macrophage cells
Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation
NLRP3 Inflammasome Sequential Changes in?Staphylococcus aureus-Induced Mouse Model of Acute Rhinosinusitis
Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Int J Mol Sci. 2012;13(7):8379-87. Doi: 10.3390/Ijms13078379. Epub 2012 Jul 5. Astragalus Membranaceus Inhibits Inflammation Via Phospho-P38 Mitogen-Activated Protein Kinase (Mapk) And Nuclear Factor (Nf)-...
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-IL-1 beta Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for macrophage using anti-IL-1 beta antibody A00101-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-02
Question
We bought anti-IL-1 beta antibody for IP on monocyte in the past. I am using human, and I plan to use the antibody for WB next. I am interested in examining monocyte as well as skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
K. Dhar
Verified customer
Asked: 2019-11-11
Answer
I took a look at the website and datasheets of our anti-IL-1 beta antibody and it appears that A00101-2 has been tested on human in both IP and WB. Thus A00101-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-11
Question
My question regarding product A00101-2, anti-IL-1 beta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00101-2 anti-IL-1 beta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-18
Question
Would anti-IL-1 beta antibody A00101-2 work for IP with macrophage?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
According to the expression profile of macrophage, IL1B is highly expressed in macrophage. So, it is likely that anti-IL-1 beta antibody A00101-2 will work for IP with macrophage.
Boster Scientific Support
Answered: 2019-09-18
Question
Do you have a BSA free version of anti-IL-1 beta antibody A00101-2 available?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-IL-1 beta antibody A00101-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-20
Question
I was wanting to use your anti-IL-1 beta antibody for IP for human macrophage on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human macrophage identification?
G. Edwards
Verified customer
Asked: 2018-09-03
Answer
You can see on the product datasheet, A00101-2 anti-IL-1 beta antibody has been tested for IP, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human macrophage in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-03
Question
Is a blocking peptide available for product anti-IL-1 beta antibody (A00101-2)?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
We do provide the blocking peptide for product anti-IL-1 beta antibody (A00101-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-04
Question
We are currently using anti-IL-1 beta antibody A00101-2 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
The anti-IL-1 beta antibody (A00101-2) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-12
Question
I see that the anti-IL-1 beta antibody A00101-2 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-10
Question
We have seen staining in human macrophage. What should we do? Is anti-IL-1 beta antibody supposed to stain macrophage positively?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
According to literature macrophage does express IL1B. According to Uniprot.org, IL1B is expressed in smooth muscle tissue, leukocyte, histiocytic lymphoma, monocyte, lung, skin, macrophage, among other tissues. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2017-05-31
Question
I am interested in to test anti-IL-1 beta antibody A00101-2 on human macrophage for research purposes, then I may be interested in using anti-IL-1 beta antibody A00101-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Li
Verified customer
Asked: 2016-09-01
Answer
The products we sell, including anti-IL-1 beta antibody A00101-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-09-01
Question
My team were satisfied with the WB result of your anti-IL-1 beta antibody. However we have observed positive staining in skin cytosol using this antibody. Is that expected? Could you tell me where is IL1B supposed to be expressed?
M. Zhao
Verified customer
Asked: 2014-10-29
Answer
According to literature, skin does express IL1B. Generally IL1B expresses in cytoplasm, cytosol. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2014-10-29
Question
I have attached the WB image, lot number and protocol we used for macrophage using anti-IL-1 beta antibody A00101-2. Please let me know if you require anything else.
K. Anderson
Verified customer
Asked: 2014-06-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-06-10
Question
Does A00101-2 anti-IL-1 beta antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Kulkarni
Verified customer
Asked: 2014-03-18
Answer
You can see on the product datasheet, A00101-2 anti-IL-1 beta antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-03-18
