Anti-EN1 (Engrailed 1) Antibody (N-term)

Product Name

Anti-EN1 (Engrailed 1) Antibody (N-term)

View all EN1/Engrailed 1 Antibodies

SKU/Catalog Number

A05395

Size

50 µl

Description

Boster Bio Anti-EN1 (Engrailed 1) Antibody (N-term) (Catalog # A05395). Tested in IF, IHC-P, WB application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-EN1 (Engrailed 1) Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05395)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05395 is reactive to EN1 in Human, Mouse

Applications

A05395 is guaranteed for IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

40115 Da

Background of EN1/Engrailed 1

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:2000
IHC-P: 1:25

Validation Images & Assay Conditions

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Fluorescent image of U251 cell stained with EN1 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0. 1%, 10 min), then incubated with EN1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin at 37℃). EN1 immunoreactivity is localized to Nucleus significantly.

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (Human Sarcoma cell line) cells labeling HME1 with A05395 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Immunofluorescence image showing nucleus and nucleoli staining on U-2 OS cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin at 1/100 dilution (red).

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All lanes : Anti-EN1 (Engrailed 1) Antibody (N-term) at 1:2000 dilution
Lane 1: U-87 MG whole cell lysate
Lane 2: U-2OS whole cell lysate
Lane 3: SH-SY5Y whole cell lysate
Lane 4: 293T/17 whole cell lysate
Lane 5: HepG2 whole cell lysate
Lane 6: Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 40 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term) (Cat. #A05395), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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A05395 staining EN1 in Human tonsil tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Gene/Protein Information For EN1 (Source: Uniprot.org, NCBI)

Alternative Names

engrailed homeobox 1; engrailed homolog 1; Homeobox protein en-1; homeobox protein engrailed-1; hu-En-1 EN1 ENDOVESLB engrailed homeobox 1 homeobox protein engrailed-1|engrailed homolog 1|homeobox protein en-1|hu-En-1

For more info on EN1, check out the EN1 Infographic

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In this infographic, you will see the following information for EN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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