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Product Info Summary
| SKU: | A09645 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PRDM12 Antibody
SKU/Catalog Number
A09645
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PRDM12 Antibody (Catalog # A09645). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRDM12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09645)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human PRDM12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09645 is reactive to PRDM12 in Human, Mouse
Reconstitution
Calculated molecular weight
40403 MW
Background of PRDM12
May be involved in transcriptional regulation.
Yang X.-H., Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09645 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HUVEC cells and A549 cells, using PRDM12 antibody A09645.
Protein Target Info & Infographic
Gene/Protein Information For PRDM12 (Source: Uniprot.org, NCBI)
Gene Name
PRDM12
Full Name
PR domain zinc finger protein 12
Weight
40403 MW
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
PR domain zinc finger protein 12;2.1.1.-;PR domain-containing protein 12;PRDM12;PFM9; PRDM12 HSAN8, PFM9 PR/SET domain 12 PR domain zinc finger protein 12|PR domain containing 12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRDM12, check out the PRDM12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRDM12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRDM12 Antibody (A09645)
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No publications found for A09645
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-PRDM12 Antibody
Question
Would anti-PRDM12 antibody A09645 work for WB with vastus lateralis?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
According to the expression profile of vastus lateralis, PRDM12 is highly expressed in vastus lateralis. So, it is likely that anti-PRDM12 antibody A09645 will work for WB with vastus lateralis.
Boster Scientific Support
Answered: 2019-12-23
Question
We are currently using anti-PRDM12 antibody A09645 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
The anti-PRDM12 antibody (A09645) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-09
Question
Will A09645 anti-PRDM12 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
It shows on the product datasheet, A09645 anti-PRDM12 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-20
Question
See below the WB image, lot number and protocol we used for vastus lateralis using anti-PRDM12 antibody A09645. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-17
Question
Can you help my question with product A09645, anti-PRDM12 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09645 anti-PRDM12 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-10
Question
Does anti-PRDM12 antibody A09645 work on monkey WB with vastus lateralis?
R. Wu
Verified customer
Asked: 2017-01-23
Answer
Our lab technicians have not tested anti-PRDM12 antibody A09645 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-PRDM12 antibody A09645 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey vastus lateralis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-23
