Anti-EMI1 (Early Mitotic Inhibitor-1) Monoclonal Antibody

Product Name

Anti-EMI1 (Early Mitotic Inhibitor-1) Monoclonal Antibody

View all EMI1 Antibodies

SKU/Catalog Number

M05229

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-EMI1 (Early Mitotic Inhibitor-1) Monoclonal Antibody (Catalog # M05229). Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-EMI1 (Early Mitotic Inhibitor-1) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05229)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: EMI1/1176

Isotype

IgG2a, kappa

Immunogen

Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M05229 is reactive to FBXO5 in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

50146 MW

Background of EMI1

This monoclonal antibody recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M05229 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml for 60 minutes at RT)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For FBXO5 (Source: Uniprot.org, NCBI)

Alternative Names

F-box only protein 5;Early mitotic inhibitor 1;FBXO5;EMI1, FBX5; FBXO5 EMI1, FBX5, Fbxo31 F-box protein 5 F-box only protein 5|F-box protein Fbx5|early mitotic inhibitor 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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