Click image to see more details
Product Info Summary
| SKU: | M00714-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Emerin Rabbit Monoclonal Antibody
SKU/Catalog Number
M00714-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Emerin Rabbit Monoclonal Antibody catalog # M00714-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Emerin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00714-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACGA-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Emerin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00714-1 is reactive to EMD in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
37 kDa
Calculated molecular weight
37337 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00714-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Emerin expression in 293T cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For EMD (Source: Uniprot.org, NCBI)
Gene Name
EMD
Full Name
Emerin
Weight
37337 MW
Alternative Names
Induced myeloid leukemia cell differentiation protein Mcl-1;Bcl-2-like protein 3;Bcl2-L-3;Bcl-2-related protein EAT/mcl1;mcl1/EAT;MCL1;BCL2L3; EMD EDMD, LEMD5, STA emerin emerin|LEM domain containing 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EMD, check out the EMD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EMD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Emerin Rabbit Monoclonal Antibody (M00714-1)
Hello CJ!
No publications found for M00714-1
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Emerin Rabbit Monoclonal Antibody
Question
I have attached the WB image, lot number and protocol we used for teratocarcinoma using anti-Emerin Monoclonal antibody M00714-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-23
Question
Do you have a BSA free version of anti-Emerin Monoclonal antibody M00714-1 available?
M. Huang
Verified customer
Asked: 2020-02-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Emerin Monoclonal antibody M00714-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-07
Question
Will M00714-1 anti-Emerin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
You can see on the product datasheet, M00714-1 anti-Emerin Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-12
Question
We have observed staining in rat placenta. What should we do? Is anti-Emerin Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
From what I have seen in literature placenta does express EMD. From what I have seen in Uniprot.org, EMD is expressed in esophagogastric junction muscularis propria, teratocarcinoma, placenta, platelet, cervix carcinoma embryonic kidney, leukemic t-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have EMD expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Embryonic kidney, Pubmed ID: 9673989
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Teratocarcinoma, Pubmed ID: 7894480
Boster Scientific Support
Answered: 2019-08-13
Question
We want to test anti-Emerin Monoclonal antibody M00714-1 on human teratocarcinoma for research purposes, then I may be interested in using anti-Emerin Monoclonal antibody M00714-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The products we sell, including anti-Emerin Monoclonal antibody M00714-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-09
Question
Is a blocking peptide available for product anti-Emerin Monoclonal antibody (M00714-1)?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
We do provide the blocking peptide for product anti-Emerin Monoclonal antibody (M00714-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-17
Question
I was wanting to use your anti-Emerin Monoclonal antibody for IF for human teratocarcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human teratocarcinoma identification?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
As indicated on the product datasheet, M00714-1 anti-Emerin Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human teratocarcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-19
Question
I see that the anti-Emerin Monoclonal antibody M00714-1 works with IF, what is the protocol used to produce the result images on the product page?
T. Krishna
Verified customer
Asked: 2018-05-09
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-09
Question
Our lab used your anti-Emerin Monoclonal antibody for WB on cervix carcinoma in the past. I am using human, and We are going to use the antibody for IF next. Our lab want to know about examining cervix carcinoma as well as teratocarcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
J. Jha
Verified customer
Asked: 2018-01-17
Answer
I took a look at the website and datasheets of our anti-Emerin Monoclonal antibody and it seems that M00714-1 has been tested on human in both WB and IF. Thus M00714-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-01-17
Question
My colleagues were satisfied with the WB result of your anti-Emerin Monoclonal antibody. However we have seen positive staining in cervix carcinoma embryonic kidney nucleus inner membrane using this antibody. Is that expected? Could you tell me where is EMD supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
According to literature, cervix carcinoma embryonic kidney does express EMD. Generally EMD expresses in nucleus inner membrane. Regarding which tissues have EMD expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Embryonic kidney, Pubmed ID: 9673989
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Teratocarcinoma, Pubmed ID: 7894480
Boster Scientific Support
Answered: 2018-01-02
Question
Can you help my question with product M00714-1, anti-Emerin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00714-1 anti-Emerin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for teratocarcinoma using anti-Emerin Monoclonal antibody M00714-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-14
Question
We are currently using anti-Emerin Monoclonal antibody M00714-1 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
C. Anderson
Verified customer
Asked: 2015-11-12
Answer
The anti-Emerin Monoclonal antibody (M00714-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-12
Question
Will anti-Emerin Monoclonal antibody M00714-1 work for IF with teratocarcinoma?
W. Miller
Verified customer
Asked: 2015-06-10
Answer
According to the expression profile of teratocarcinoma, EMD is highly expressed in teratocarcinoma. So, it is likely that anti-Emerin Monoclonal antibody M00714-1 will work for IF with teratocarcinoma.
Boster Scientific Support
Answered: 2015-06-10
Question
Is this M00714-1 anti-Emerin Monoclonal antibody reactive to the isotypes of EMD?
R. Krishna
Verified customer
Asked: 2014-06-06
Answer
The immunogen of M00714-1 anti-Emerin Monoclonal antibody is A synthesized peptide derived from human Emerin Emerin may regulate gene expression through binding to other transcriptional regulators. Emerin binds to Beta -catenin and inhibits its nuclear accumulation. Recent studies demonstrate that Emerin is required for HIV-1 infectivity. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-06-06
