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Product Info Summary
| SKU: | A03840-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Elovl4 Antibody Picoband®
SKU/Catalog Number
A03840-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Elovl4 Antibody Picoband® catalog # A03840-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Elovl4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03840-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Elovl4 recombinant protein (Position: M1-E312).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03840-1 is reactive to Elovl4 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
127459 MW
Background of ELOVL4
Elongation of very long chain fatty acids protein 4 is a protein that in humans is encoded by the ELOVL4 gene. This gene encodes a membrane-bound protein which is a member of the ELO family, proteins which participate in the biosynthesis of fatty acids. Consistent with the expression of the encoded protein in photoreceptor cells of the retina, mutations and small deletions in this gene are associated with Stargardt-like macular dystrophy (STGD3) and autosomal dominant Stargardt-like macular dystrophy (ADMD), also referred to as autosomal dominant atrophic macular degeneration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03840-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat C6 whole cell, mouse NIH/3T3 whole cell
ICC/IF: RM-1 cell
FCM: HEPA1-6 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Elovl4 using anti-Elovl4 antibody (A03840-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat C6 whole cell lysates,
Lane 2: mouse NIH/3T3 whole cell lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Elovl4 antigen affinity purified polyclonal antibody (Catalog # A03840-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Elovl4 at approximately 37 kDa. The expected band size for Elovl4 is at 37 kDa.
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Figure 2. IF analysis of Elovl4 using anti-Elovl4 antibody (A03840-1).
Elovl4 was detected in an immunocytochemical section of RM-1 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Elovl4 Antibody (A03840-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of HEPA1-6 cells using anti-Elovl4 antibody (A03840-1).
Overlay histogram showing HEPA1-6 cells stained with A03840-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Elovl4 Antibody (A03840-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Elovl4 (Source: Uniprot.org, NCBI)
Gene Name
Elovl4
Full Name
Elongation of very long chain fatty acids protein 4
Weight
127459 MW
Superfamily
ELO family
Alternative Names
AF4/FMR2 family member 4;ALL1-fused gene from chromosome 5q31 protein;Protein AF-5q31;Major CDK9 elongation factor-associated protein;AFF4;AF5Q31, MCEF;HSPC092; ELOVL4 ADMD, CT118, ISQMR, SCA34, STGD2, STGD3 ELOVL fatty acid elongase 4 elongation of very long chain fatty acids protein 4|3-keto acyl-CoA synthase ELOVL4|ELOVL FA elongase 4|cancer/testis 118|elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4|very long chain 3-ketoacyl-CoA synthase 4|very long chain 3-oxoacyl-CoA synthase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Elovl4, check out the Elovl4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Elovl4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Elovl4 Antibody Picoband® (A03840-1)
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