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Product Info Summary
| SKU: | A02141-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Elongation factor 1-alpha 1 eEF1A1 Antibody
SKU/Catalog Number
A02141-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Elongation factor 1-alpha 1 eEF1A1 Antibody catalog # A02141-1. Tested in WB,ICC,IF,IHC,Flow Cytometry,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Elongation factor 1-alpha 1 eEF1A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02141-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02141-1 is reactive to EEF1A1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
50141 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02141-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of EEF1A1 using Anti-Elongation factor 1-alpha 1 eEF1A1 Antibody (A02141-1).
ICC staining eEF1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-Elongation factor 1-alpha 1 eEF1A1 Antibody A02141-1
Western blot analysis of eEF1A1 on different lysates using anti-eEF1A1 antibody at 1/500 dilution.Positive control:
Lane 1: Rat brain tissue
Lane 2: Mouse skeletal muscle
Lane 3: Mouse cerebellum
Lane 4: Rat skin
Lane 5: Daudi
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For EEF1A1 (Source: Uniprot.org, NCBI)
Gene Name
EEF1A1
Full Name
Elongation factor 1-alpha 1
Weight
50141 MW
Superfamily
TRAFAC class translation factor GTPase superfamily
Alternative Names
Elongation factor 1-alpha 1;EF-1-alpha-1;Elongation factor Tu;EF-Tu;Eukaryotic elongation factor 1 A-1;eEF1A-1;Leukocyte receptor cluster member 7;EEF1A1;EEF1A, EF1A, LENG7; EEF1A1 CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, GRAF-1EF, LENG7, PTI1, eEF1A-1 eukaryotic translation elongation factor 1 alpha 1 elongation factor 1-alpha 1|CTCL tumor |EF-1-alpha-1|EF1a-like protein|cervical cancer suppressor 3|elongation factor 1 alpha subunit|elongation factor Tu|epididymis secretory sperm binding protein|eukaryotic elongation factor 1 A-1|eukaryotic translation elongation factor 1 alpha 1-like 14|glucocorticoid receptor AF-1 specific elongation factor|leukocyte receptor cluster (LRC) member 7|leukocyte receptor cluster member 7|prostate tumor-inducing protein 1|translation elongation factor 1 alpha 1-like 14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EEF1A1, check out the EEF1A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EEF1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Elongation factor 1-alpha 1 eEF1A1 Antibody (A02141-1)
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