Anti-Egr1 Antibody Picoband®

EGR1 antibody

Boster Bio Anti-Egr1 Antibody catalog # PA2177. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 2 publication(s).

Product Info Summary

SKU: PA2177
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Egr1 Antibody Picoband®

View all EGR1 Antibodies

SKU/Catalog Number

PA2177

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Egr1 Antibody catalog # PA2177. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Egr1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2177)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Egr1, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2177 is reactive to EGR1 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

58 kDa

Calculated molecular weight

57507 MW

Background of EGR1

Earlu growth response protein 1, also known as ZIF268 or NGFIA, is a protein that in humans is encoded by the EGR1 gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. This gene is mapped to 5q31.2. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. It has a distinct pattern of expression in the brain, and its induction has been shown to be associated with neuronal activity. Several studies suggest it has a role in neuronal plasticity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2177 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For EGR1 (Source: Uniprot.org, NCBI)

Gene Name

EGR1

Full Name

Early growth response protein 1

Weight

57507 MW

Superfamily

EGR C2H2-type zinc-finger protein family

Alternative Names

Early growth response protein 1;EGR-1;AT225;Nerve growth factor-induced protein A;NGFI-A;Transcription factor ETR103;Transcription factor Zif268;Zinc finger protein 225;Zinc finger protein Krox-24;EGR1;KROX24, ZNF225; EGR1 AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225 early growth response 1 early growth response protein 1|EGR-1|nerve growth factor-induced protein A|transcription factor ETR103|transcription factor Zif268|zinc finger protein 225|zinc finger protein Krox-24

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EGR1, check out the EGR1 Infographic

EGR1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EGR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA2177 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Identification, Characterization, and Regulatory Mechanisms of a Novel EGR1 Splicing Isoform

Lin K, Fang S, Cai B, Huang X, Zhang X, Lu Y, Zhang W, Wei E. Eur J Cell Biol. 2014 Jul;93(7):278-88. Doi: 10.1016/J.Ejcb.2014.05.001. Epub 2014 May 20. Erk/Egr-1 Signaling Pathway Is Involved In Cyslt2 Receptor-Mediated Il-8 Production In Hek293 ...

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5 Customer Q&As for Anti-Egr1 Antibody Picoband®

Question

I was wanting to use your anti-Egr1 antibody for WB for human pns on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human pns identification?

Verified Customer

Verified customer

Asked: 2020-05-04

Answer

As indicated on the product datasheet, PA2177 anti-Egr1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human pns in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-05-04

Question

Is this PA2177 anti-Egr1 antibody reactive to the isotypes of EGR1?

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

The immunogen of PA2177 anti-Egr1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human EGR1(23-43aa FPHSPTMDNYPKLEEMMLLSN), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-20

Question

We are currently using anti-Egr1 antibody PA2177 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

The anti-Egr1 antibody (PA2177) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-15

Question

Does PA2177 anti-Egr1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

You can see on the product datasheet, PA2177 anti-Egr1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-21

Question

I see that the anti-Egr1 antibody PA2177 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-11-28

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-11-28

Order DetailsPrice
PA2177

100μg

$370
PA2177-10ug

10μg sample (liquid)

$99
PA2177-Biotin

100 μg Biotin conjugated

$570
PA2177-Cy3

100 μg Cy3 conjugated

$570
PA2177-Dylight488

100 μg Dylight488 conjugated

$570
PA2177-Dylight550

100 μg Dylight550 conjugated

$570
PA2177-Dylight594

100 μg Dylight594 conjugated

$570
PA2177-FITC

100 μg FITC conjugated

$570
PA2177-HRP

100 μg HRP conjugated

$570
PA2177-APC

100 μg APC conjugated

$670
PA2177-PE

100 μg PE conjugated

$670
PA2177-iFluor647

100 μg iFluor647 conjugated

$670
PA2177-carrier-free

Carrier Free

$370

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PA2177
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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