Product Info Summary
SKU: | A12840-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-EGFL4 (R1274) MEGF8 Antibody
SKU/Catalog Number
A12840-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-EGFL4 (R1274) MEGF8 Antibody catalog # A12840-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EGFL4 (R1274) MEGF8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12840-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human GPR61 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12840-1 is reactive to MEGF8 in Human, Mouse, Rat
Applications
A12840-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
303100 MW
Background of MEGF8
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Triepels R.H.,
Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Mao M., Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
![a12840 1 anti egfl4 r1274 megf8 antibody ihc testing 2 a12840 1 anti egfl4 r1274 megf8 antibody ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a12840-1-anti-egfl4-r1274-megf8-antibody-ihc-testing-2.jpg)
Click image to see more details
Figure 2. Immunohistochemistry validation of MEGF8 using Anti-EGFL4 (R1274) MEGF8 Antibody (A12840-1).
Immunohistochemistry (IHC) analyzes of EGFL4 (R1274) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.For more protocol information of IHC
![a12840 1 anti egfl4 r1274 megf8 antibody wb testing 1 a12840 1 anti egfl4 r1274 megf8 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a12840-1-anti-egfl4-r1274-megf8-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-EGFL4 (R1274) MEGF8 Antibody A12840-1
Western blot (WB) analysis of EGFL4 (R1274) pAb at 1:500 dilutionLane1:L02 whole cell lysate(40ug)
Lane2:SGC7901 whole cell lysate(40ug)
Lane3:C6 whole cell lysate(40ug)
Lane4:AML-12 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MEGF8 (Source: Uniprot.org, NCBI)
Gene Name
MEGF8
Full Name
Multiple epidermal growth factor-like domains protein 8
Weight
303100 MW
Alternative Names
C19orf49; chromosome 19 open reading frame 49; EGFL4MGC120684; EGF-like domain-containing protein 4; EGF-like protein 4; EGF-like-domain, multiple 4; Epidermal growth factor-like protein 4; FLJ22365; HBV pre-s2 binding protein 1; KIAA0817; MGC138147; multiple EGF-like domain protein 4; Multiple EGF-like domains protein 8; multiple EGF-like-domains 8; multiple epidermal growth factor-like domains 8; multiple epidermal growth factor-like domains protein 8; SBP1 MEGF8 C19orf49, CRPT2, EGFL4, SBP1 multiple EGF like domains 8 multiple epidermal growth factor-like domains protein 8|EGF-like domain-containing protein 4|EGF-like-domain, multiple 4|HBV pre-S2-binding protein 1|HBV pre-s2 binding protein 1|epidermal growth factor-like protein 4|hepatitis B virus pre-S2-binding protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MEGF8, check out the MEGF8 Infographic
![MEGF8 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MEGF8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EGFL4 (R1274) MEGF8 Antibody (A12840-1)
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