Anti-EF-Ts/TSFM Antibody Picoband™

Product Name

Anti-EF-Ts/TSFM Antibody Picoband™

View all TSFM Antibodies

SKU/Catalog Number

A08656-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EF-Ts/TSFM Antibody Picoband™ catalog # A08656-2. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-EF-Ts/TSFM Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08656-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human EF-Ts/TSFM recombinant protein (Position: M1-E325).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08656-2 is reactive to TSFM in Human

Applications

A08656-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

36881 MW

Background of TSFM

Elongation factor Ts, mitochondrial is a protein that in humans is encoded by the TSFM gene. It is an EF-Ts homolog. This gene encodes a mitochondrial translation elongation factor. The encoded protein is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in this gene are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of EF-Ts/TSFM using anti-EF-Ts/TSFM antibody (A08656-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EF-Ts/TSFM antigen affinity purified polyclonal antibody (Catalog # A08656-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EF-Ts/TSFM at approximately 35 kDa. The expected band size for EF-Ts/TSFM is at 35 kDa.

Gene/Protein Information For TSFM (Source: Uniprot.org, NCBI)

Alternative Names

COXPD3; EFTS; EF-Ts; EFTSMT; EF-Tsmt; mitochondrial elongation factor Ts; mitochondrial; Ts translation elongation factor, mitochondrial TSFM EFTS, EFTSMT Ts translation elongation factor, mitochondrial elongation factor Ts, mitochondrial|mitochondrial elongation factor Ts

For more info on TSFM, check out the TSFM Infographic

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In this infographic, you will see the following information for TSFM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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