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Product Info Summary
| SKU: | A08656-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-EF-Ts/TSFM Antibody Picoband®
SKU/Catalog Number
A08656-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EF-Ts/TSFM Antibody Picoband® catalog # A08656-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-EF-Ts/TSFM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08656-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human EF-Ts/TSFM recombinant protein (Position: M1-E325).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08656-2 is reactive to TSFM in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
36881 MW
Background of TSFM
Elongation factor Ts, mitochondrial is a protein that in humans is encoded by the TSFM gene. It is an EF-Ts homolog. This gene encodes a mitochondrial translation elongation factor. The encoded protein is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in this gene are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08656-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human 293T whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EF-Ts/TSFM using anti-EF-Ts/TSFM antibody (A08656-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EF-Ts/TSFM antigen affinity purified polyclonal antibody (Catalog # A08656-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EF-Ts/TSFM at approximately 35 kDa. The expected band size for EF-Ts/TSFM is at 35 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TSFM (Source: Uniprot.org, NCBI)
Gene Name
TSFM
Full Name
Elongation factor Ts, mitochondrial
Weight
36881 MW
Superfamily
EF-Ts family
Alternative Names
Annexin A8 ;Annexin VIII ;Annexin-8 ;Vascular anticoagulant-beta ;VAC-beta ;ANXA8 ;ANX8; TSFM EFTS, EFTSMT Ts translation elongation factor, mitochondrial elongation factor Ts, mitochondrial|mitochondrial elongation factor Ts
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSFM, check out the TSFM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSFM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EF-Ts/TSFM Antibody Picoband® (A08656-2)
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