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Product Info Summary
| SKU: | PB9191 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-EDA Antibody Picoband®
SKU/Catalog Number
PB9191
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EDA Antibody Picoband® catalog # PB9191. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EDA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9191)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human EDA recombinant protein (Position: A30-S391). Human EDA shares 95% amino acid (aa) sequence identity with mouse EDA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9191 is reactive to EDA in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
41294 MW
Background of EDA
Ectodysplasin-A is a protein that in humans is encoded by the EDA gene. It is mapped to Xq13.1. The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9191 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Colo320 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EDA using anti-EDA antibody (PB9191).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Colo320 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDA antigen affinity purified polyclonal antibody (Catalog # PB9191) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDA at approximately 41 kDa. The expected band size for EDA is at 41 kDa.
Click image to see more details
Figure 2. Western blot analysis of EDA using anti-EDA antibody (PB9191).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: recombinant human EDA protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDA antigen affinity purified polyclonal antibody (Catalog # PB9191) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDA at approximately 43 kDa. The expected band size for EDA is at 43 kDa.
Protein Target Info & Infographic
Gene/Protein Information For EDA (Source: Uniprot.org, NCBI)
Gene Name
EDA
Full Name
Ectodysplasin-A
Weight
41294 MW
Superfamily
tumor necrosis factor family
Alternative Names
Ectodysplasin-A;Ectodermal dysplasia protein;EDA protein;Ectodysplasin-A, membrane form;Ectodysplasin-A, secreted form;EDA;ED1, EDA2; EDA ECTD1, ED1, ED1-A1, ED1-A2-A1, EDA-A2, EDA1, EDA2, HED, HED1, ODT1, STHAGX1, TNLG7C, XHED, XLHED, EDA ectodysplasin A ectodysplasin-A|X-linked anhidroitic ectodermal dysplasia protein|oligodontia 1|tumor necrosis factor ligand 7C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EDA, check out the EDA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EDA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EDA Antibody Picoband® (PB9191)
Hello CJ!
PB9191 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
X-irradiation for inhibiting glial scar formation in injured spinal cord
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Customer Q&As
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1 Customer Q&As for Anti-EDA Antibody Picoband®
Question
We are currently using anti-EDA antibody PB9191 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
B. Mitchell
Verified customer
Asked: 2014-05-30
Answer
The anti-EDA antibody (PB9191) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-05-30
