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Product Info Summary
| SKU: | A01113-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-EDA Antibody (N-term)
SKU/Catalog Number
A01113-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-EDA Antibody (N-term) (Catalog # A01113-1). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-EDA Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01113-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This EDA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-76 amino acids from the N-terminal region of human EDA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01113-1 is reactive to EDA in Human
Reconstitution
Calculated molecular weight
41294 Da
Background of EDA
EDA is a type II membrane protein that can be cleaved by furin to produce a secreted form. This protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in the gene for EDA are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01113-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysate from RD cell line,using EDA Antibody (N-term).A01113-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EDA antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of hela cells using EDA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For EDA (Source: Uniprot.org, NCBI)
Gene Name
EDA
Full Name
Ectodysplasin-A
Weight
41294 Da
Superfamily
tumor necrosis factor family
Alternative Names
Ectodysplasin-A, Ectodermal dysplasia protein, EDA protein, Ectodysplasin-A, membrane form, Ectodysplasin-A, secreted form, EDA, ED1, EDA2 EDA ECTD1, ED1, ED1-A1, ED1-A2-A1, EDA-A2, EDA1, EDA2, HED, HED1, ODT1, STHAGX1, TNLG7C, XHED, XLHED, EDA ectodysplasin A ectodysplasin-A|X-linked anhidroitic ectodermal dysplasia protein|oligodontia 1|tumor necrosis factor ligand 7C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EDA, check out the EDA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EDA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EDA Antibody (N-term) (A01113-1)
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