Anti-ECSIT Antibody

Product Name

Anti-ECSIT Antibody

View all Ecsit Antibodies

SKU/Catalog Number

A07966

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ECSIT Antibody (Catalog # A07966). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.

Storage & Handling

ECSIT antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ECSIT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07966)

Host

Rabbit

Contents

ECSIT Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ECSIT antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human ECSIT. The immunogen is located within amino acids 290 - 340 of ECSIT.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Multiple isoforms of CAPS2 are known to exist. This CAPS2 antibody is predicted to be specific to CAPS2 and not recognize CAPS1.

Reactive Species

A07966 is reactive to ECSIT in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

49148 MW

Background of Ecsit

Activation of NF-κB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07966 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ECSIT antibody can be used for detection of ECSIT by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For ECSIT (Source: Uniprot.org, NCBI)

Alternative Names

SITPEC, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, Protein SITPEC Ecsit|Sit, Sitpec|ECSIT signalling integrator|evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial|ECSIT homolog|signaling intermediate in Toll pathway-evolutionarily conserved

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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