Product Info Summary
SKU: | M02861-1 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ECM1 Rabbit Monoclonal Antibody
View all ECM-1/Secretory Component P85 Antibodies
SKU/Catalog Number
M02861-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-ECM1 Rabbit Monoclonal Antibody catalog # M02861-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ECM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02861-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
25E30
Isotype
IgG
Immunogen
A synthesized peptide derived from human ECM1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02861-1 is reactive to ECM1 in Human, Mouse, Rat
Applications
M02861-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
25762 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
![m02861 1 ecm1 primary antibodies wb testing 1 m02861 1 ecm1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m02861-1-ecm1-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of ECM1 using anti-ECM1 antibody (M02861-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECM1 antigen affinity purified monoclonal antibody (Catalog # M02861-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECM1 at approximately 75 kDa. The expected band size for ECM1 is at 61 kDa.
![m02861 1 ecm1 primary antibodies wb testing 2 m02861 1 ecm1 primary antibodies wb testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m02861-1-ecm1-primary-antibodies-wb-testing-2.jpg)
Click image to see more details
Figure 2. Western blot analysis of ECM1 using anti-ECM1 antibody (M02861-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat skin tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECM1 antigen affinity purified monoclonal antibody (Catalog # M02861-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECM1 at approximately 75 kDa. The expected band size for ECM1 is at 61 kDa.
![m02861 1 ecm1 primary antibodies ihc testing 3 m02861 1 ecm1 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m02861-1-ecm1-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of ECM1 using anti-ECM1 antibody (M02861-1).
ECM1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ECM1 Antibody (M02861-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![m02861 1 ecm1 primary antibodies ihc testing 4 m02861 1 ecm1 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/m/0/m02861-1-ecm1-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of ECM1 using anti-ECM1 antibody (M02861-1).
ECM1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ECM1 Antibody (M02861-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![m02861 1 ecm1 primary antibodies ihc testing 5 m02861 1 ecm1 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/m/0/m02861-1-ecm1-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of ECM1 using anti-ECM1 antibody (M02861-1).
ECM1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ECM1 Antibody (M02861-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![m02861 1 ecm1 primary antibodies ihc testing 6 m02861 1 ecm1 primary antibodies ihc testing 6](https://www.bosterbio.com/media/catalog/product/m/0/m02861-1-ecm1-primary-antibodies-ihc-testing-6.jpg)
Click image to see more details
Figure 6. IHC analysis of ECM1 using anti-ECM1 antibody (M02861-1).
ECM1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ECM1 Antibody (M02861-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ECM1 (Source: Uniprot.org, NCBI)
Gene Name
ECM1
Full Name
Extracellular matrix protein 1
Weight
25762 MW
Alternative Names
ECM1; extracellular matrix protein 1; p85; Secretory Component Glycoprotein; Secretory Component P85 ECM1 URBWD extracellular matrix protein 1 extracellular matrix protein 1|secretory component p85|testicular tissue protein Li 61
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ECM1, check out the ECM1 Infographic
![ECM1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ECM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ECM1 Rabbit Monoclonal Antibody (M02861-1)
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