Anti-IgA Secretory Component / ECM1 Monoclonal Antibody

Product Name

Anti-IgA Secretory Component / ECM1 Monoclonal Antibody

View all ECM-1/Secretory Component P85 Antibodies

SKU/Catalog Number

M02861

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-IgA Secretory Component / ECM1 Monoclonal Antibody (Catalog # M02861). Tested in Flow Cytometry, IF applications. This antibody reacts with Human, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-IgA Secretory Component / ECM1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02861)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: ECM1/792

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human ECM1 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02861 is reactive to ECM1 in Human, Rat

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

60674 MW

Background of ECM-1/Secretory Component P85

This monoclonal antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02861 is guaranteed for Flow Cytometry, IF Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For ECM1 (Source: Uniprot.org, NCBI)

Alternative Names

Extracellular matrix protein 1;Secretory component p85;ECM1; ECM1 URBWD extracellular matrix protein 1 extracellular matrix protein 1|secretory component p85|testicular tissue protein Li 61

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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