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Product Info Summary
| SKU: | M04204 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-DP1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04204
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-DP1 Rabbit Monoclonal Antibody catalog # M04204. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04204)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
24T75
Isotype
IgG
Immunogen
A synthesized peptide derived from human DP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04204 is reactive to TFDP1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
366649 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04204 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50
Positive Control
WB: human Raji whole cell, human A431 whole cell, human THP-1 whole cell, human 293T whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse RAW2647 whole cell, mouse Neuro-2a whole cell
IHC: human breast cancer tissue, human colorectal adenocarcinoma tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DP1 using anti-DP1 antibody (M04204).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DP1 antigen affinity purified monoclonal antibody (Catalog # M04204) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DP1 at approximately 50 kDa. The expected band size for DP1 is at 45 kDa.
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Figure 2. IHC analysis of DP1 using anti-DP1 antibody (M04204).
DP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DP1 Antibody (M04204) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of DP1 using anti-DP1 antibody (M04204).
DP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DP1 Antibody (M04204) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TFDP1 (Source: Uniprot.org, NCBI)
Gene Name
TFDP1
Full Name
Transcription factor Dp-1
Weight
366649 MW
Superfamily
E2F/DP family
Alternative Names
Laminin subunit alpha-3;Epiligrin 170 kDa subunit;E170;Epiligrin subunit alpha;Kalinin subunit alpha;Laminin-5 subunit alpha;Laminin-6 subunit alpha;Laminin-7 subunit alpha;Nicein subunit alpha;LAMA3;LAMNA; TFDP1 DILC, DP1, DRTF1, Dp-1 transcription factor Dp-1 transcription factor Dp-1|DRTF1-polypeptide 1|E2F dimerization partner 1|E2F-related transcription factor|down-regulated in liver cancer stem cells
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TFDP1, check out the TFDP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TFDP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DP1 Rabbit Monoclonal Antibody (M04204)
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