Click image to see more details
Product Info Summary
| SKU: | A09019-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Docking protein 3 DOK3 Antibody
SKU/Catalog Number
A09019-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Docking protein 3 DOK3 Antibody (Catalog # A09019-1). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Docking protein 3 DOK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09019-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human DOK3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09019-1 is reactive to DOK3 in Human, Mouse
Reconstitution
Calculated molecular weight
53288 MW
Background of Dok3
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function By similarity.
Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Favre C., Genes Immun. 4:40-45(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09019-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HepG2 cells, using DOK3 antibody A09019-1.
Protein Target Info & Infographic
Gene/Protein Information For DOK3 (Source: Uniprot.org, NCBI)
Gene Name
DOK3
Full Name
Docking protein 3
Weight
53288 MW
Superfamily
DOK family
Alternative Names
Docking protein 3;Downstream of tyrosine kinase 3;DOK3; Dok3|AI450713, Dokl|docking protein 3|docking protein 3|DOK-L|Dok-like protein|downstream of tyrosine kinase 3|p62(dok)-like protein|p62Dok-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DOK3, check out the DOK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DOK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Docking protein 3 DOK3 Antibody (A09019-1)
Hello CJ!
No publications found for A09019-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Docking protein 3 DOK3 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Docking protein 3 DOK3 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Docking protein 3 DOK3 Antibody
Question
We are currently using anti-DOK3 antibody A09019-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The anti-DOK3 antibody (A09019-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-24
Question
I am looking for to test anti-DOK3 antibody A09019-1 on human erythroleukemia for research purposes, then I may be interested in using anti-DOK3 antibody A09019-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
The products we sell, including anti-DOK3 antibody A09019-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-07
Question
Will anti-DOK3 antibody A09019-1 work on bovine WB with blood?
R. Johnson
Verified customer
Asked: 2015-08-07
Answer
Our lab technicians have not validated anti-DOK3 antibody A09019-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-DOK3 antibody A09019-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-07
Question
I see that the anti-DOK3 antibody A09019-1 works with WB, what is the protocol used to produce the result images on the product page?
S. Anderson
Verified customer
Asked: 2014-10-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-10-28
Question
I have a question about product A09019-1, anti-DOK3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Zhang
Verified customer
Asked: 2014-08-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09019-1 anti-DOK3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-08-27
