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Product Info Summary
| SKU: | A08512 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-DNase II DNASE2 Antibody
SKU/Catalog Number
A08512
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DNase II DNASE2 Antibody (Catalog # A08512). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
DNase II antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-DNase II DNASE2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08512)
Host
Rabbit
Contents
DNase II Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DNase II antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DNase II. The immunogen is located within the last 50 amino acids of DNase II.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
MYH8 antibody is human, mouse and rat reactive. MYH8 antibody is predicted to not cross-react with other members of the myosin heavy chain family.
Reactive Species
A08512 is reactive to DNASE2 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
39581 MW
Background of DNase II
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08512 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DNase II in human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase antibody II at 1:500 dilution.
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Immunohistochemistry of DNaseII in human spleen tissue with DNaseII antibody at 5 μg/mL.
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Immunofluorescence of DNase II in human spleen tissue with DNase II antibody at 5 μg/ml.
Green: DNase II antibody (A08512)
Red: Phylloidin staining
Blue: DAPI staining
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Immunohistochemistry of DNase II in human spleen tissue with DNase II antibody at 5 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For DNASE2 (Source: Uniprot.org, NCBI)
Gene Name
DNASE2
Full Name
Deoxyribonuclease-2-alpha
Weight
39581 MW
Superfamily
DNase II family
Alternative Names
DNL, DNL2, DNASE2A, Deoxyribonuclease-2-alpha, Acid DNase, DNase II alpha DNASE2 DNASE2A, DNL, DNL2 deoxyribonuclease 2, lysosomal deoxyribonuclease-2-alpha|DNase II alpha|DNase II, lysosomal|R31240_2|acid DNase|deoxyribonuclease II alpha|deoxyribonuclease II, lysosomal|lysosomal DNase II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNASE2, check out the DNASE2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNASE2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNase II DNASE2 Antibody (A08512)
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