Product Info Summary
SKU: | A06835-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-DnaJB4 Antibody
SKU/Catalog Number
A06835-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DnaJB4 Antibody catalog # A06835-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DnaJB4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06835-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DNAJB4. AA range:91-140
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06835-1 is reactive to DNAJB4 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
37807 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06835-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using DnaJB4 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For DNAJB4 (Source: Uniprot.org, NCBI)
Gene Name
DNAJB4
Full Name
DnaJ homolog subfamily B member 4
Weight
37807 MW
Alternative Names
DnaJ homolog subfamily B member 4;Heat shock 40 kDa protein 1 homolog;HSP40 homolog;Heat shock protein 40 homolog;Human liver DnaJ-like protein;DNAJB4;DNAJW, HLJ1; DNAJB4 DNAJW, DjB4, HLJ1 DnaJ heat shock protein family (Hsp40) member B4 dnaJ homolog subfamily B member 4|DnaJ (Hsp40) homolog, subfamily B, member 4|DnaJ-like heat shock protein 40|HSP40 homolog|heat shock 40 kDa protein 1 homolog|heat shock protein 40 homolog|human liver DnaJ-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNAJB4, check out the DNAJB4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNAJB4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DnaJB4 Antibody (A06835-1)
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No publications found for A06835-1
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Customer Q&As
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5 Customer Q&As for Anti-DnaJB4 Antibody
Question
My lab would like to test anti-DnaJB4 antibody A06835-1 on human lymph for research purposes, then I may be interested in using anti-DnaJB4 antibody A06835-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
The products we sell, including anti-DnaJB4 antibody A06835-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-26
Question
Will A06835-1 anti-DnaJB4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
As indicated on the product datasheet, A06835-1 anti-DnaJB4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-25
Question
We are currently using anti-DnaJB4 antibody A06835-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
The anti-DnaJB4 antibody (A06835-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-06
Question
I was wanting to use your anti-DnaJB4 antibody for WB for human lymph on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymph identification?
C. Jha
Verified customer
Asked: 2018-11-21
Answer
It shows on the product datasheet, A06835-1 anti-DnaJB4 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lymph in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-21
Question
My question regarding product A06835-1, anti-DnaJB4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
W. Li
Verified customer
Asked: 2013-11-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06835-1 anti-DnaJB4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-11-14