Anti-DNA Ligase I (E139) LIG1 Antibody

Product Name

Anti-DNA Ligase I (E139) LIG1 Antibody

View all DNA Ligase I Antibodies

SKU/Catalog Number

A02380

Size

100ul

Form

Liquid

Description

Boster Bio Anti-DNA Ligase I (E139) LIG1 Antibody catalog # A02380. Tested in WB,IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DNA Ligase I (E139) LIG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02380)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic peptide from human EYA1/EYA4 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02380 is reactive to LIG1 in Human

Applications

A02380 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

101736 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200

Validation Images & Assay Conditions

Click image to see more details

Figure 2. Immunohistochemistry validation of LIG1 using Anti-DNA Ligase I (E139) LIG1 Antibody (A02380).

Immunohistochemistry (IHC) analyzes of DNA Ligase I (E139) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.
For more protocol information of IHC

Click image to see more details

Figure 1. Western blotting validation for Anti-DNA Ligase I (E139) LIG1 Antibody A02380

Western blot (WB) analysis of DNA Ligase I (E139) pAb at 1:500 dilution
Lane1:K562 whole cell lysate(40ug)
Lane2:L02 whole cell lysate(40ug)
Lane3:Jurkat whole cell lysate(40ug)
Lane4:A549 whole cell lysate(10ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For LIG1 (Source: Uniprot.org, NCBI)

Alternative Names

DNA ligase 1; DNA ligase I; EC 6.5.1.1; ligase I, DNA, ATP-dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1; polydeoxyribonucleotide synthase ATP 1 LIG1 DNA ligase 1 DNA ligase 1|ligase I, DNA, ATP-dependent|polydeoxyribonucleotide synthase [ATP] 1

For more info on LIG1, check out the LIG1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LIG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the LIG1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis