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Product Info Summary
| SKU: | A04923 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-DHX36 Antibody
SKU/Catalog Number
A04923
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DHX36 Antibody (Catalog # A04923). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.
Storage & Handling
DHX36 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-DHX36 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04923)
Host
Rabbit
Contents
DHX36 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal DHX36 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DHX36. The immunogen is located within the last 50 amino acids of DHX36.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of DDX36 are known to exist; this antibody will detect the three longest isoforms.
Reactive Species
A04923 is reactive to Dhx36 in Human, Mouse
Applications
A04923 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
114760 MW
Background of Dhx36
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure. DHX36 (DEAH box protein 36), also known as MLE-like protein 1 and RNA helicase associated with AU-rich element ARE (RHAU), belongs to RNA helicase of the DEAH family and may function in sex development and spermatogenesis. It is expressed in testis and is evolutionary conserved with true orthologs in almost all animal species. DHX36 plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. DHX36 is required for early embryogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DHX36 antibody can be used for detection of DHX36 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DHX36 in mouse liver tissue lysate with DHX36 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of DHX36 in human liver tissue with DHX36 antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Dhx36 (Source: Uniprot.org, NCBI)
Gene Name
Dhx36
Full Name
ATP-dependent DNA/RNA helicase DHX36
Weight
114760 MW
Superfamily
DEAD box helicase family
Alternative Names
DDX36; DEAH (Asp-Glu-Ala-His) box polypeptide 36; DEAH box protein 36; EC 3.6.1; EC 3.6.4.13; G4 resolvase-1; KIAA1488G4R1; MLEL1RHAUDEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 36; MLE-like protein 1; probable ATP-dependent RNA helicase DHX36; RNA helicase associated with AU-rich element ARE Dhx36|2810407E23Rik, AI452301, AU022184, Ddx36, mKIAA1488|DEAH (Asp-Glu-Ala-His) box polypeptide 36|ATP-dependent DNA/RNA helicase DHX36|ATP-dependent RNA helicase DHX36|DEAD/H box polypeptide 36|DEAH box protein 36|MLE-like protein 1|RNA helicase associated with AU-rich element ARE|probable ATP-dependent RNA helicase DHX36
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Dhx36, check out the Dhx36 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Dhx36: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DHX36 Antibody (A04923)
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