Product Info Summary
SKU: | A07077-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-Diacylglycerol kinase eta DGKH Antibody
SKU/Catalog Number
A07077-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Diacylglycerol kinase eta DGKH Antibody (Catalog# A07077-1). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Diacylglycerol kinase eta DGKH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07077-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human DGKH. Immunogen sequence location: 771-820
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07077-1 is reactive to DGKH in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
134866 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07077-1 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using DGKH Antibody. The picture on the right is blocked with the synthesized peptide.
Click image to see more details
Immunofluorescence analysis of HeLa cells, using DGKH Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For DGKH (Source: Uniprot.org, NCBI)
Gene Name
DGKH
Full Name
Diacylglycerol kinase eta
Weight
134866 MW
Superfamily
eukaryotic diacylglycerol kinase family
Alternative Names
DGKH;Diacylglycerol kinase eta;DAG kinase eta;Diglyceride kinase eta;DGK-eta DGKH DGKeta diacylglycerol kinase eta diacylglycerol kinase eta|DAG kinase eta|diglyceride kinase eta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DGKH, check out the DGKH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DGKH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Diacylglycerol kinase eta DGKH Antibody (A07077-1)
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No publications found for A07077-1
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Customer Q&As
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5 Customer Q&As for Anti-Diacylglycerol kinase eta DGKH Antibody
Question
We are currently using anti-DGKH antibody A07077-1 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
The anti-DGKH antibody (A07077-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-16
Question
We are interested in to test anti-DGKH antibody A07077-1 on human testis for research purposes, then I may be interested in using anti-DGKH antibody A07077-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The products we sell, including anti-DGKH antibody A07077-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-20
Question
Is this A07077-1 anti-DGKH antibody reactive to the isotypes of DGKH?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The immunogen of A07077-1 anti-DGKH antibody is A peptide derived from human DGKH. Immunogen sequence location: 771-820. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-09
Question
Is a blocking peptide available for product anti-DGKH antibody (A07077-1)?
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
We do provide the blocking peptide for product anti-DGKH antibody (A07077-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-DGKH antibody A07077-1. Let me know if you need anything else.
D. Miller
Verified customer
Asked: 2014-09-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-09-17