Anti-DGKE/Dgk Epsilon Antibody

DGK-epsilon antibody

Boster Bio Anti-DGKE/Dgk Epsilon Antibody (Catalog # A06615). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A06615
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-DGKE/Dgk Epsilon Antibody

View all DGK-epsilon Antibodies

SKU/Catalog Number

A06615

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-DGKE/Dgk Epsilon Antibody (Catalog # A06615). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DGKE/Dgk Epsilon Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06615)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human DGKE.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06615 is reactive to DGKE in Human, Mouse, Rat

Applications

A06615 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

63927 MW

Background of DGK-epsilon

Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.

Tang W., J. Biol. Chem. 271:10237-10241(1996).
Tang W., Gene 239:185-192(1999).
Sjoeblom T., Science 314:268-274(2006).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For DGKE (Source: Uniprot.org, NCBI)

Gene Name

DGKE

Full Name

Diacylglycerol kinase epsilon

Weight

63927 MW

Superfamily

eukaryotic diacylglycerol kinase family

Alternative Names

DAG kinase epsilon; DAGK5; DAGK6; DGK; DGKE; DGKepsilon; DGK-epsilon; diacylglycerol kinase epsilon; diacylglycerol kinase, epsilon (64kD); diacylglycerol kinase, epsilon 64kDa; Diglyceride kinase epsilon; EC 2.7.1.107 DGKE AHUS7, DAGK5, DAGK6, DGK, NPHS7 diacylglycerol kinase epsilon diacylglycerol kinase epsilon|DAG kinase epsilon|DGK-epsilon|diacylglycerol kinase, epsilon 64kDa|diglyceride kinase epsilon

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DGKE, check out the DGKE Infographic

DGKE infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DGKE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-DGKE/Dgk Epsilon Antibody

Question

Does anti-DGKE/Dgk Epsilon antibody A06615 work for WB with prostate?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

According to the expression profile of prostate, DGKE is highly expressed in prostate. So, it is likely that anti-DGKE/Dgk Epsilon antibody A06615 will work for WB with prostate.

Boster Scientific Support

Answered: 2019-07-04

Question

Is this A06615 anti-DGKE/Dgk Epsilon antibody reactive to the isotypes of DGKE?

K. Roberts

Verified customer

Asked: 2017-07-04

Answer

The immunogen of A06615 anti-DGKE/Dgk Epsilon antibody is Synthesized peptide derived from internal of human DGKE. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-07-04

Question

We are currently using anti-DGKE/Dgk Epsilon antibody A06615 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

A. Thomas

Verified customer

Asked: 2016-11-22

Answer

The anti-DGKE/Dgk Epsilon antibody (A06615) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-11-22

Order DetailsPrice
A06615-100uL

100uL

$360

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A06615
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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