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Product Info Summary
| SKU: | MA1020 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ICC, WB |
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Product info
Product Name
Anti-CDK4 Antibody (Monoclonal, DCS-31)
SKU/Catalog Number
MA1020
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CDK4 Antibody (Monoclonal, DCS-31) catalog # MA1020. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK4 Antibody (Monoclonal, DCS-31) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1020)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
DCS-31
Isotype
Mouse IgG2a
Immunogen
Recombinant human Cdk4 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1020 is reactive to CDK4 in Human, Mouse, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
34 kDa
Calculated molecular weight
44225 MW
Background of CDK4
Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1020 is guaranteed for ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
Positive Control
WB: human Hela whole cell, human K562 whole cell, rat PC-12 whole cell, mosue NIH/3T3 whole cell, mosue RAW2647 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDK4 using anti-CDK4 antibody (MA1020).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mosue NIH/3T3 whole cell lysates,
Lane 5: mosue RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CDK4 antigen affinity purified monoclonal antibody (Catalog # MA1020) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CDK4 at approximately 34 kDa. The expected band size for CDK4 is at 34 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CDK4 (Source: Uniprot.org, NCBI)
Gene Name
CDK4
Full Name
Cyclin-dependent kinase 4
Weight
44225 MW
Superfamily
protein kinase superfamily
Alternative Names
CDK4; Cell division protein kinase 4; CMM3; cyclin-dependent kinase 4; EC 2.7.11; EC 2.7.11.22; melanoma cutaneous malignant, 3; MGC14458; PSK-J3; PSK-J3cell division kinase 4 CDK4 CMM3, PSK-J3 cyclin dependent kinase 4 cyclin-dependent kinase 4|cell division protein kinase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK4, check out the CDK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDK4 Antibody (Monoclonal, DCS-31) (MA1020)
Hello CJ!
MA1020 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
MA1020 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000
Effect of Bone Morphogenetic Protein-2 on Proliferation and Apoptosis of Gastric Cancer Cells
Liao S, Xiao S, Chen H, Zhang M, Chen Z, Long Y, Gao L, He J, Ge Y, Yi W, Wu M, Li G, Zhou Y. Int J Oncol. 2017 Nov;51(5):1497-1507. doi: 10.3892/ijo.2017.4137. Epub 2017 Sep 27. The receptor for activated protein kinase C promotes cell growth, in...
Liu W, Dai Q, Lu N, Wei L, Ha J, Rong J, Mu R, You Q, Li Z, Guo Q. Biochem Cell Biol. 2011 Jun;89(3):287-98. Doi: 10.1139/O10-162. Epub 2011 Apr 14. Lyg-202 Inhibits The Proliferation Of Human Colorectal Carcinoma Hct-116 Cells Through Induction O...
Wang Hy, Huang Rp, Han P, Xue Db, Li Hb, Liu B, Shan P, Wang Qs, Li Ks, Li Hl. Cell Biochem Funct. 2014 Mar;32(2):201-8. Doi: 10.1002/Cbf.2995. Epub 2013 Sep 17. The Effects Of Artemisinin On The Proliferation And Apoptosis Of Vascular Smooth Musc...
Li Bh, Xu Sb, Li F, Zou Xg, Saimaiti A, Simayi D, Wang Yh, Zhang Y, Yuan J, Zhang Wj. Cell Signal. 2012 Mar;24(3):718-25. Doi: 10.1016/J.Cellsig.2011.11.005. Epub 2011 Nov 13. Stat6 Activity-Related Th2 Cytokine Profile And Tumor Growth Advantage ...
Zhang H, Zhao B, Huang C, Meng Xm, Bian Eb, Li J. Plos One. 2014 May 2;9(5):E95520. Doi: 10.1371/Journal.Pone.0095520. Ecollection 2014. Melittin Restores Pten Expression By Down-Regulating Hdac2 In Human Hepatocelluar Carcinoma Hepg2 Cells.
Han W, Liu Gn. Life Sci. 2010 Feb 13;86(7-8):234-43. Doi: 10.1016/J.Lfs.2009.12.005. Epub 2009 Dec 16. Egr-1 Decoy Odns Inhibit Vascular Smooth Muscle Cell Proliferation And Neointimal Hyperplasia Of Balloon-Injured Arteries In Rat.
Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.
Zhou R, Lu Z, Liu K, Guo J, Liu J, Zhou Y, Yang J, Mi M, Xu H. Curr Cancer Drug Targets. 2015;14(9):860-71. Platycodin D Induces Tumor Growth Arrest By Activating Foxo3A Expression In Prostate Cancer In Vitro And In Vivo.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CDK4 Antibody (Monoclonal, DCS-31)
Question
Will anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 work for ICC with right ovary?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
According to the expression profile of right ovary, CDK4 is highly expressed in right ovary. So, it is likely that anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 will work for ICC with right ovary.
Boster Scientific Support
Answered: 2020-05-06
Question
We were well pleased with the WB result of your anti-CDK4 antibody (Monoclonal, DCS-31). However we have been able to see positive staining in muscle cytoplasm. using this antibody. Is that expected? Could you tell me where is CDK4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
According to literature, muscle does express CDK4. Generally CDK4 expresses in cytoplasm. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-23
Question
Do you have a BSA free version of anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 available?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-13
Question
Can you help my question with product MA1020, anti-CDK4 antibody (Monoclonal, DCS-31). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1020 anti-CDK4 antibody (Monoclonal, DCS-31), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-03
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-CDK4 antibody (Monoclonal, DCS-31) MA1020. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-10
Question
Is a blocking peptide available for product anti-CDK4 antibody (Monoclonal, DCS-31) (MA1020)?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
We do provide the blocking peptide for product anti-CDK4 antibody (Monoclonal, DCS-31) (MA1020). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-17
Question
Is this MA1020 anti-CDK4 antibody (Monoclonal, DCS-31) reactive to the isotypes of CDK4?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The immunogen of MA1020 anti-CDK4 antibody (Monoclonal, DCS-31) is Recombinant human Cdk4 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-15
Question
We are currently using anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
The anti-CDK4 antibody (Monoclonal, DCS-31) (MA1020) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-31
Question
We purchased anti-CDK4 antibody (Monoclonal, DCS-31) for ICC on embryo a few years ago. I am using rat, and We intend to use the antibody for WB next. I am interested in examining embryo as well as right ovary in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
I took a look at the website and datasheets of our anti-CDK4 antibody (Monoclonal, DCS-31) and I see that MA1020 has been validated on rat in both ICC and WB. Thus MA1020 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-25
Question
I was wanting to use your anti-CDK4 antibody (Monoclonal, DCS-31) for ICC for rat right ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat right ovary identification?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
As indicated on the product datasheet, MA1020 anti-CDK4 antibody (Monoclonal, DCS-31) has been tested for ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat right ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-17
Question
Does MA1020 anti-CDK4 antibody (Monoclonal, DCS-31) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Huang
Verified customer
Asked: 2019-02-22
Answer
It shows on the product datasheet, MA1020 anti-CDK4 antibody (Monoclonal, DCS-31) as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-22
Question
We have observed staining in mouse right ovary. What should we do? Is anti-CDK4 antibody (Monoclonal, DCS-31) supposed to stain right ovary positively?
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
From literature right ovary does express CDK4. From Uniprot.org, CDK4 is expressed in right ovary, embryo, muscle, among other tissues. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-05-11
Question
We are interested in to test anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 on rat right ovary for research purposes, then I may be interested in using anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
The products we sell, including anti-CDK4 antibody (Monoclonal, DCS-31) MA1020, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-18
Question
Here is the WB image, lot number and protocol we used for right ovary using anti-CDK4 antibody (Monoclonal, DCS-31) MA1020. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-02
Question
I see that the anti-CDK4 antibody (Monoclonal, DCS-31) MA1020 works with ICC, what is the protocol used to produce the result images on the product page?
K. Patel
Verified customer
Asked: 2016-05-24
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-05-24
