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Product Info Summary
| SKU: | A07973-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-DGK- beta Antibody
SKU/Catalog Number
A07973-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DGK- beta Antibody catalog # A07973-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DGK- beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07973-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DGKB. AA range:657-706
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07973-1 is reactive to DGKB in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
90595 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07973-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western blot analysis of HuvEC cells using DGK-Beta Polyclonal Antibody
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Western blot analysis of the lysates from HUVECcells using DGKB antibody.
Protein Target Info & Infographic
Gene/Protein Information For DGKB (Source: Uniprot.org, NCBI)
Gene Name
DGKB
Full Name
Diacylglycerol kinase beta
Weight
90595 MW
Superfamily
eukaryotic diacylglycerol kinase family
Alternative Names
Diacylglycerol kinase beta;DAG kinase beta;2.7.1.107;90 kDa diacylglycerol kinase;Diglyceride kinase beta;DGK-beta;DGKB;DAGK2, KIAA0718; DGKB DAGK2, DGK, DGK-BETA diacylglycerol kinase beta diacylglycerol kinase beta|90 kDa diacylglycerol kinase|DAG kinase beta|diacylglycerol kinase, beta 90kDa|diglyceride kinase beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DGKB, check out the DGKB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DGKB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DGK- beta Antibody (A07973-1)
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Customer Q&As
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5 Customer Q&As for Anti-DGK- beta Antibody
Question
I have a question about product A07973-1, anti-DGK- beta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Patel
Verified customer
Asked: 2020-02-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07973-1 anti-DGK- beta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-28
Question
I have attached the WB image, lot number and protocol we used for caudate nucleus using anti-DGK- beta antibody A07973-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-19
Question
We are currently using anti-DGK- beta antibody A07973-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
The anti-DGK- beta antibody (A07973-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-01
Question
Is a blocking peptide available for product anti-DGK- beta antibody (A07973-1)?
H. Johnson
Verified customer
Asked: 2017-12-26
Answer
We do provide the blocking peptide for product anti-DGK- beta antibody (A07973-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-26
Question
I was wanting to use your anti-DGK- beta antibody for WB for mouse caudate nucleus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse caudate nucleus identification?
Verified Customer
Verified customer
Asked: 2017-08-31
Answer
As indicated on the product datasheet, A07973-1 anti-DGK- beta antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse caudate nucleus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-31
