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Product Info Summary
| SKU: | M03093 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Desmoglein-3 (Squamous Cell Marker) DSG3 Monoclonal Antibody
View all Desmoglein-3 Antibodies
SKU/Catalog Number
M03093
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Desmoglein-3 (Squamous Cell Marker) DSG3 Monoclonal Antibody (Catalog # M03093). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Desmoglein-3 (Squamous Cell Marker) DSG3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03093)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: DSG3/1535
Isotype
IgG1, kappa
Immunogen
Recombinant human DSG3 extracellular protein fragment
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M03093 is reactive to DSG3 in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
107533 MW
Background of Desmoglein-3
Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This monoclonal antibody is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80%) and specificity (100%) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03093 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Lung Squamous Cell Carcinoma stained with Anti-Desmoglein-3 Monoclonal Antibody (DSG3/1535).
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Formalin-fixed, paraffin-embedded human Skin stained with Anti-Desmoglein-3 Monoclonal Antibody (DSG3/1535).
Protein Target Info & Infographic
Gene/Protein Information For DSG3 (Source: Uniprot.org, NCBI)
Gene Name
DSG3
Full Name
Desmoglein-3
Weight
107533 MW
Alternative Names
Desmoglein-3;130 kDa pemphigus vulgaris antigen;PVA;Cadherin family member 6;DSG3;CDHF6; DSG3 ABOLM, CDHF6, PVA desmoglein 3 desmoglein-3|130 kDa pemphigus vulgaris |cadherin family member 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DSG3, check out the DSG3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DSG3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Desmoglein-3 (Squamous Cell Marker) DSG3 Monoclonal Antibody (M03093)
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Customer Q&As
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15 Customer Q&As for Anti-Desmoglein-3 (Squamous Cell Marker) DSG3 Monoclonal Antibody
Question
We have observed staining in human tongue. Are there any suggestions? Is anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody supposed to stain tongue positively?
A. Miller
Verified customer
Asked: 2020-03-12
Answer
From what I have seen in literature tongue does express DSG3. From what I have seen in Uniprot.org, DSG3 is expressed in mouth mucosa, tongue, saliva, among other tissues. Regarding which tissues have DSG3 expression, here are a few articles citing expression in various tissues:
Saliva, Pubmed ID: 16740002
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-12
Question
Will anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093 work on pig IHC with tongue?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
Our lab technicians have not validated anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig tongue in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-06
Question
I was wanting to use your anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody for IHC for human saliva on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human saliva identification?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
As indicated on the product datasheet, M03093 anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human saliva in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-03
Question
Does anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093 work for IHC with saliva?
R. Wu
Verified customer
Asked: 2019-12-03
Answer
According to the expression profile of saliva, DSG3 is highly expressed in saliva. So, it is likely that anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093 will work for IHC with saliva.
Boster Scientific Support
Answered: 2019-12-03
Question
I have attached the WB image, lot number and protocol we used for saliva using anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-10
Question
Is this M03093 anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody reactive to the isotypes of DSG3?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The immunogen of M03093 anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody is Recombinant human DSG3 extracellular protein fragment (exact sequence is proprietary). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-22
Question
Our lab were happy with the WB result of your anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody. However we have seen positive staining in saliva cell membrane using this antibody. Is that expected? Could you tell me where is DSG3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
From what I have seen in literature, saliva does express DSG3. Generally DSG3 expresses in cell membrane. Regarding which tissues have DSG3 expression, here are a few articles citing expression in various tissues:
Saliva, Pubmed ID: 16740002
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-20
Question
I see that the anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-11
Question
We want to test anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093 on human saliva for research purposes, then I may be interested in using anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Parker
Verified customer
Asked: 2017-01-31
Answer
The products we sell, including anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-01-31
Question
Is there a BSA free version of anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093 available?
B. Johnson
Verified customer
Asked: 2015-10-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-10-26
Question
My question regarding product M03093, anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Brown
Verified customer
Asked: 2015-07-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03093 anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-07-24
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for saliva using anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093. Let me know if you need anything else.
A. Bhatt
Verified customer
Asked: 2015-04-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-07
Question
Is a blocking peptide available for product anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody (M03093)?
G. Kulkarni
Verified customer
Asked: 2014-09-15
Answer
We do provide the blocking peptide for product anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody (M03093). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-09-15
Question
Does M03093 anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Edwards
Verified customer
Asked: 2014-07-02
Answer
It shows on the product datasheet, M03093 anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-07-02
Question
We are currently using anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody M03093 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
E. Li
Verified customer
Asked: 2014-02-20
Answer
The anti-Desmoglein-3 (Squamous Cell Marker) Monoclonal antibody (M03093) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-02-20
