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Product Info Summary
| SKU: | PA1567 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Desmoglein 3/DSG3 Antibody Picoband®
View all Desmoglein-3 Antibodies
SKU/Catalog Number
PA1567
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Desmoglein 3/DSG3 Antibody catalog # PA1567. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Desmoglein 3/DSG3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1567)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Desmoglein 3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1567 is reactive to DSG3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
130 kDa
Calculated molecular weight
107533 MW
Background of Desmoglein-3
Desmoglein3 is also known as PVA (Pemphigus Vulgaris Antigen). Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are autoimmune diseases of the skin which have as target antigens 2 different members of the desmoglein subfamily of the desmosomal cadherins: pemphigus vulgaris antigen (PVA, or desmoglein-3) in the case of PV and desmoglein I in the case of PF. The DSG3 gene, like the genes for DSG1 and DSG2, is located on chromosome 18. The human DSG3 gene consists of 15 exons and spans more than 23 kb. DSG3 and DSG1 are the autoantigens of pemphigus vulgaris and pemphigus foliaceus, respectively. DSG3 and DSG1 are the target antigens in PNP, and IgG autoantibodies against DSG3 in PNP sera play a pathogenic role in the loss of keratinocyte adhesion and blistering.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1567 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human A431 whole cell, human Hacat whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DSG3 using anti-DSG3 antibody (PA1567).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DSG3 antigen affinity purified polyclonal antibody (Catalog # PA1567) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DSG3 at approximately 130 kDa. The expected band size for DSG3 is at 107 kDa.
Protein Target Info & Infographic
Gene/Protein Information For DSG3 (Source: Uniprot.org, NCBI)
Gene Name
DSG3
Full Name
Desmoglein-3
Weight
107533 MW
Alternative Names
Desmoglein-3;130 kDa pemphigus vulgaris antigen;PVA;Cadherin family member 6;DSG3;CDHF6; DSG3 ABOLM, CDHF6, PVA desmoglein 3 desmoglein-3|130 kDa pemphigus vulgaris |cadherin family member 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DSG3, check out the DSG3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DSG3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Desmoglein 3/DSG3 Antibody Picoband® (PA1567)
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Customer Q&As
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4 Customer Q&As for Anti-Desmoglein 3/DSG3 Antibody Picoband®
Question
We have seen staining in human tongue. What should we do? Is anti-Desmoglein 3/DSG3 antibody supposed to stain tongue positively?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
According to literature tongue does express DSG3. According to Uniprot.org, DSG3 is expressed in mouth mucosa, tongue, saliva, among other tissues. Regarding which tissues have DSG3 expression, here are a few articles citing expression in various tissues:
Saliva, Pubmed ID: 16740002
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-13
Question
I would like using your anti-Desmoglein 3/DSG3 antibody for homophilic cell adhesion via plasma membrane adhesion molecules studies. Has this antibody been tested with western blotting on human a431 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Thank you for your inquiry. This PA1567 anti-Desmoglein 3/DSG3 antibody is validated on human a431 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-29
Question
My team were content with the WB result of your anti-Desmoglein 3/DSG3 antibody. However we have been able to see positive staining in mouth mucosa cell membrane using this antibody. Is that expected? Could you tell me where is DSG3 supposed to be expressed?
K. Jha
Verified customer
Asked: 2015-06-08
Answer
According to literature, mouth mucosa does express DSG3. Generally DSG3 expresses in cell membrane. Regarding which tissues have DSG3 expression, here are a few articles citing expression in various tissues:
Saliva, Pubmed ID: 16740002
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-06-08
Question
We are currently using anti-Desmoglein 3/DSG3 antibody PA1567 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
B. Johnson
Verified customer
Asked: 2013-12-30
Answer
The anti-Desmoglein 3/DSG3 antibody (PA1567) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-12-30
