Anti-Desmoglein-1 (DSG1) Monoclonal Antibody

Product Name

Anti-Desmoglein-1 (DSG1) Monoclonal Antibody

View all DSG1 Antibodies

SKU/Catalog Number

M02655

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Desmoglein-1 (DSG1) Monoclonal Antibody (Catalog # M02655). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Desmoglein-1 (DSG1) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02655)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: DSG1/1733

Isotype

IgG1, kappa

Immunogen

Recombinant human desmoglein-1 protein fragment corresponding to intracellular domain

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02655 is reactive to DSG1 in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

113748 MW

Background of DSG1

Recognizes a protein of ~150kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family.Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms.Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02655 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For DSG1 (Source: Uniprot.org, NCBI)

Alternative Names

Desmoglein-1;Cadherin family member 4;Desmosomal glycoprotein 1;DG1;DGI;Pemphigus foliaceus antigen;DSG1;CDHF4; DSG1 CDHF4, DG1, DSG, EPKHE, EPKHIA, PPKS1, SPPK1 desmoglein 1 desmoglein-1|cadherin family member 4|desmosomal glycoprotein 1|pemphigus foliaceus

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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