Anti-DDAH1 Monoclonal Antibody

Product Name

Anti-DDAH1 Monoclonal Antibody

View all DDAH1 Antibodies

SKU/Catalog Number

M03136

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-DDAH1 Monoclonal Antibody catalog # M03136. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DDAH1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03136)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEAG-4

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human DDAH1 Hydrolyzes N (G),N (G) -dimethyl-L-arginine (ADMA) and N (G) -monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03136 is reactive to DDAH1 in Human, Monkey, Mouse, Rat

Applications

M03136 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

191615 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
FC 1:30

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of DDAH1 using anti-DDAH1 antibody (M03136).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDAH1 antigen affinity purified monoclonal antibody (Catalog # M03136) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDAH1 at approximately 37 kDa. The expected band size for DDAH1 is at 31 kDa.

Gene/Protein Information For DDAH1 (Source: Uniprot.org, NCBI)

Alternative Names

DDAH1; DDAH-1; DDAHdimethylargininase-1; DDAHI; Dimethylargininase-1; dimethylarginine dimethylaminohydrolase 1FLJ25539; EC 3.5.3.18; FLJ21264; N(G); N(G)-dimethylarginine dimethylaminohydrolase 1; NG, NG-dimethylarginine dimethylaminohydrolase DDAH1 DDAH, DDAH-1, DDAHI, HEL-S-16 dimethylarginine dimethylaminohydrolase 1 N(G),N(G)-dimethylarginine dimethylaminohydrolase 1|NG, NG-dimethylarginine dimethylaminohydrolase|dimethylargininase-1|epididymis secretory protein Li 16

For more info on DDAH1, check out the DDAH1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DDAH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the DDAH1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis