Anti-DBF4 Antibody Picoband™

Product Name

Anti-DBF4 Antibody Picoband™

View all DBF4 Antibodies

SKU/Catalog Number

A01348-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DBF4 Antibody Picoband™ catalog # A01348-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DBF4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01348-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human DBF4 recombinant protein (Position:Y295-S665).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01348-2 is reactive to DBF4 in Human, Mouse, Rat

Applications

A01348-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

Background of DBF4

Using both gain- and loss-of-function experiments, it was found that Xenopus Dbf4 inhibited canonical Wnt signaling. Depletion of endogenous Dbf4 did not disturb gastrulation movements or early organizer genes, but resulted in embryos with morphologically defective heart and eyes and suppressed cardiac markers. The function of Dbf4 in heart development appeared to be independent of its role in the cell cycle. Dbf4 interacted physically and functionally with Frodo, a context-dependent regulator of Wnt signaling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry(Fixed), 1-3μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of DBF4 using anti-DBF4 antibody (A01348-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBF4 antigen affinity purified polyclonal antibody (Catalog # A01348-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DBF4 at approximately 80 kDa. The expected band size for DBF4 is at 80 kDa.

Click image to see more details

Figure 2. IF analysis of DBF4 using anti-DBF4 antibody (A01348-2) and anti-Beta Tubulin antibody (M01857-3).
DBF4 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DBF4 Antibody (A01348-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Click image to see more details

Figure 3. Flow Cytometry analysis of Raji cells using anti-DBF4 antibody (A01348-2).
Overlay histogram showing Raji cells stained with A01348-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBF4 Antibody (A01348-2, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For DBF4 (Source: Uniprot.org, NCBI)

Alternative Names

ASKchif; DBF4 homolog (S. cerevisiae); DBF4ACHIF; DBF4-type zinc finger-containing protein 1; ZDBF1DBF-type containing 1 DBF4 ASK, CHIFA, ZDBF1, DBF4 DBF4 zinc finger protein DBF4 homolog A|DBF4 homolog|DBF4 zinc finger A|DBF4-type zinc finger-containing protein 1|activator of S phase kinase|chiffon homolog A|zinc finger, DBF-type containing 1

For more info on DBF4, check out the DBF4 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DBF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the DBF4 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis