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Product Info Summary
| SKU: | A01348-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Protein DBF4 homolog A DBF4 Antibody
SKU/Catalog Number
A01348-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Protein DBF4 homolog A DBF4 Antibody (Catalog # A01348-1). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein DBF4 homolog A DBF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01348-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human DBF4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01348-1 is reactive to DBF4 in Human, Mouse
Reconstitution
Calculated molecular weight
76858 MW
Background of DBF4
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Kumagai H., Mol. Cell. Biol. 19:5083-5095(1999).
Jiang W., EMBO J. 18:5703-5713(1999).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01348-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from NIH-3T3 cells treated with H2O2 (100uM, 30mins) and COS-7 cells treated with PMA (125mg/ml, 30mins), using DBF4 antibody A01348-1.
Protein Target Info & Infographic
Gene/Protein Information For DBF4 (Source: Uniprot.org, NCBI)
Gene Name
DBF4
Full Name
Protein DBF4 homolog A
Weight
76858 MW
Alternative Names
Protein DBF4 homolog A;Activator of S phase kinase;Chiffon homolog A;DBF4-type zinc finger-containing protein 1;DBF4;ASK, DBF4A, ZDBF1; DBF4 ASK, CHIFA, ZDBF1, DBF4 DBF4 zinc finger protein DBF4 homolog A|DBF4 homolog|DBF4 zinc finger A|DBF4-type zinc finger-containing protein 1|activator of S phase kinase|chiffon homolog A|zinc finger, DBF-type containing 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DBF4, check out the DBF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DBF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein DBF4 homolog A DBF4 Antibody (A01348-1)
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No publications found for A01348-1
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Protein DBF4 homolog A DBF4 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-DBF4 antibody A01348-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-03
Question
We are currently using anti-DBF4 antibody A01348-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?
T. Bhatt
Verified customer
Asked: 2019-08-27
Answer
The anti-DBF4 antibody (A01348-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-27
Question
Is this A01348-1 anti-DBF4 antibody reactive to the isotypes of DBF4?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The immunogen of A01348-1 anti-DBF4 antibody is Synthesized peptide derived from N-terminal of human DBF4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-24
Question
See below the WB image, lot number and protocol we used for cervix carcinoma using anti-DBF4 antibody A01348-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-30
Question
I was wanting to use your anti-DBF4 antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
You can see on the product datasheet, A01348-1 anti-DBF4 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-07
Question
I see that the anti-DBF4 antibody A01348-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-25
Question
Is a blocking peptide available for product anti-DBF4 antibody (A01348-1)?
H. Williams
Verified customer
Asked: 2015-07-23
Answer
We do provide the blocking peptide for product anti-DBF4 antibody (A01348-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-07-23
