Anti-DARPP-32 (P69) PPP1R1B Antibody

Product Name

Anti-DARPP-32 (P69) PPP1R1B Antibody

View all DARPP-32 Antibodies

SKU/Catalog Number

A03424

Size

100ul

Form

Liquid

Description

Boster Bio Anti-DARPP-32 (P69) PPP1R1B Antibody catalog # A03424. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DARPP-32 (P69) PPP1R1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03424)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human LRP4 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03424 is reactive to Ppp1r1b in Human, Mouse, Rat

Applications

A03424 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

22963 MW

Background of DARPP-32

Involved in calcium binding and microtubule stabilization.

Gross B., Nucleic Acids Res. 17:8367-8367(1989).
Dunham A., Nature 428:522-528(2004).
Hochstrasser D.F., Electrophoresis 13:992-1001(1992).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Immunohistochemistry (IHC) analyzes of DARPP-32 (P69) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

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Western blot (WB) analysis of DARPP-32 (P69) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug)

Gene/Protein Information For Ppp1r1b (Source: Uniprot.org, NCBI)

Alternative Names

DARPP32; DARPP-32; PPP1R1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B; regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32) Ppp1r1b|AU040756, DARP, DARPP-32, Dar, Darpp32|protein phosphatase 1, regulatory inhibitor subunit 1B|protein phosphatase 1 regulatory subunit 1B|dopamine- and cAMP-regulated neuronal phosphoprotein

For more info on Ppp1r1b, check out the Ppp1r1b Infographic

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In this infographic, you will see the following information for Ppp1r1b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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