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Product Info Summary
| SKU: | M01161 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody
View all DAP Kinase 1 Antibodies
SKU/Catalog Number
M01161
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody catalog # M01161. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01161)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
COA-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DAP Kinase 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01161 is reactive to DAPK1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated molecular weight
160046 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01161 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: HeLa cell
IHC: human breast cancer tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of DAP Kinase 1 expression in HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using DAP Kinase 1 Antibody.
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Immunofluorescent analysis of Hela cells, using DAP Kinase 1 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For DAPK1 (Source: Uniprot.org, NCBI)
Gene Name
DAPK1
Full Name
Death-associated protein kinase 1
Weight
160046 MW
Superfamily
protein kinase superfamily
Alternative Names
Death-associated protein kinase 1;DAP kinase 1;2.7.11.1;DAPK1;DAPK; DAPK1 DAPK, ROCO3 death associated protein kinase 1 death-associated protein kinase 1|DAP kinase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DAPK1, check out the DAPK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DAPK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody (M01161)
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Customer Q&As
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6 Customer Q&As for Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody
Question
I see that the anti-DAP Kinase 1 Rabbit Monoclonal antibody M01161 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-25
Question
My question regarding product M01161, anti-DAP Kinase 1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01161 anti-DAP Kinase 1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-19
Question
Here is the WB image, lot number and protocol we used for amygdala using anti-DAP Kinase 1 Rabbit Monoclonal antibody M01161. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-16
Question
Would M01161 anti-DAP Kinase 1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
It shows on the product datasheet, M01161 anti-DAP Kinase 1 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala using anti-DAP Kinase 1 Rabbit Monoclonal antibody M01161. Let me know if you need anything else.
K. Rodriguez
Verified customer
Asked: 2015-09-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-09-04
Question
We are currently using anti-DAP Kinase 1 Rabbit Monoclonal antibody M01161 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
B. Lewis
Verified customer
Asked: 2013-02-25
Answer
The anti-DAP Kinase 1 Rabbit Monoclonal antibody (M01161) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-25
